GeneTex
United States (US)

IGF2 protein

Cat No. GTX29575

Reactivity Human
Species Human
APPLICATION

Positive Control

Recombinant hIL-11
PROPERTIES

Form

Lyophilized powder

Storage

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20 ºC. We recommend a quick spin followed by reconstitution in 10 mM acetic acid to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into oth

Antigen Species

Human

Immunogen

Highly pure (>98%) recombinant hIL-11 (human Interleukin-11)

Region/Sequence

Highly pure (>98%) recombinant hIL-11 (human Interleukin-11)

Purification


Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Insulin Like Growth Factor 2,C11Orf43,Grdf,Igf-Ii,Pp9974,Igf2

Cellular Localization

Secreted

Background

Insulin-like Growth Factor II (IGFII) was first isolated from human serum by Froesch, et al., as a factor displaying insulin like activities that were not suppressed by antibodies to insulin. It had been discovered that growth hormone dependent factors in serum stimulate the incorporation of 35S into cartilage and that calf serum factors induced cellular division in chick fibroblasts. In 1972, the term ""somatomedin"" was introduced in an unsuccessful attempt to unify the nomenclature of these hormone-dependent factors. In 1987, a consensus among an international group of scientists endorsed the use of the terms insulin like growth factors (IGFI and IGFII), originally proposed by Rinderknecht and Humbel. Hence, IGFI and IGFII have had several synomyms: nonsuppressible insulin like activity (NSIL-A), sulfation factor activity (SFA), and multiplication stimulating activity (MSA). Because IGFII was not regulated by growth hormone, only IGFI was known as a somatomedin. Human IGFII contains 67 amino acids and shares similar structural features with IGFI, including a 62% sequence homology. In human plasma, IGFI and IGFII are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGFII binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGFII is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells.

Research Area

Package List Price ($)
$ 299