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IKB alpha peptide

Cat. No. GTX27546

Application

 Neutralizing/Inhibition

Species

 Human
Package
50 μg ($189)
See all IKB alpha products

APPLICATION

Application Note

Intended for use as a control peptide when used with anti-IkB alpha (C-terminal specific ab GTX27545) to block specific interaction of anti-IkB alpha (C-terminal specific) with the IkB alpha subunit. Control peptide should be used at 1.0 mg per 1.0 ml of antiserum per assay.

PROPERTIES

Form

Liquid

Buffer

0.15M Sodium Chloride, 0.02M Potassium Phosphate pH7.2

Preservative

0.01% Sodium azide

Storage

Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Nfkb Inhibitor Alpha , Ikba , Mad-3 , Nfkbi , Nfkbia

Cellular Localization

Cytoplasmic

Background

Three major forms of IkB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkB-alpha and IkB-beta appear to be the major regulatory forms of IkB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkB and block nuclear import by masking the nuclear localization sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkB. Immunoblotting easily detects the hyper-phosphorylated forms of IkB-alpha, but not phosphorylated IkB-beta. Interestingly, IkB-alpha and IkB-beta mediate different NFkB responses. IkB-alpha appears to control more transient activation of NFkB in response to an inducer, while IkB-beta controls a persistent response. Bcl-3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IkB is confirmed by immunoblotting.

Research Area

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 189