*Optimal dilutions/concentrations should be determined by the researcher.
|1:100 - 1:200
|1:400 - 1:800
|1:500 - 1:2,000
Not tested in other applications.
Primarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions.The antiserum does not recognize human IL-1alpha.
0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
2 mg/ml (Please refer to the vial label for the specific concentration.)
This antibody was prepared by repeated immunizations with recombinant human IL-1s produced in E.coli.? The MW of the recombinant 153 aa IL-1s was 17 kDa with the N-terminal amino acid at position alanine 117.? This cleavage site is generated by the IL-1s
From polyclonal serum
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.