IL1 beta antibody

Name: IL1 beta antibody
Brand: GeneTex
SKU: GTX22105
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-IL1 beta antibody used in Western Blot (WB). GTX22105

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX22105

Cat. No. GTX22105

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP, ELISA, Neutralizing/Inhibition
Reactivity	Human, Dog, Primate
Package	100 μg ($399)

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Datasheet
Reference

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
IHC-P	1:100 - 1:200
IP	1:400 - 1:800
ELISA	1:500 - 1:2,000
Neutralizing/Inhibition	1:100

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

Primarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions.The antiserum does not recognize human IL-1alpha.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was prepared by repeated immunizations with recombinant human IL-1s produced in E.coli.? The MW of the recombinant 153 aa IL-1s was 17 kDa with the N-terminal amino acid at position alanine 117.? This cleavage site is generated by the IL-1s

Purification

IgG fraction
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_368268

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Cytoplasm, cytosol,Lysosome,Secreted

Background

Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.

Database

Gene ID: 3553 IL1B
UniProt: P01584 IL1B

Research Area

DATA IMAGES

GTX22105 WB Image

GTX22105 was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1beta by Western blot. Lane 1 and 2 were loaded with 2.5 μg and 500 ng of dog IL-1beta respectively.

GTX22105 IHC-P Image

IHC-P analysis of medullary lymph node using IL1 beta antibody. Positive staining in the cytoplasm of circulating macrophages. Negative Control (far right) normal rabbit IgG.

REFERENCE

Application Reference

Sgrignoli MR et al. Stem Cell Res 2019; 39 : 101525 Reduction in the inflammatory markers CD4, IL-1, IL-6 and TNF帢 in dogs with keratoconjunctivitis sicca treated topically with mesenchymal stem cells.

Application : IHC-P

Reactivity : Dog

Lee YC et al. Int J Mol Sci 2019; 20 (7) Essential Role of Visfatin in Lipopolysaccharide and Colon Ascendens Stent Peritonitis-Induced Acute Lung Injury.

Application : WB

Reactivity : Human

Chu Y et al. Int J Mol Sci 2019; 20 (1) Protective Effects and Mechanisms of N-Phenethyl Caffeamide from UVA-Induced Skin Damage in Human Epidermal Keratinocytes through Nrf2/HO-1 Regulation.

Application : WB

Reactivity : Human

Lai WY et al. Int J Oral Sci 2014; 6 (2) : 34547 An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements.

Application : WB

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