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IL1 beta antibody

Anti-IL1 beta antibody used in Western Blot (WB). GTX22105
Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX22105

Cat No. GTX22105

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ELISA, Neutralizing/Inhibition

Reactivity

Human, Dog, Primate
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
IHC-P 1:100 - 1:200
IP 1:400 - 1:800
ELISA 1:500 - 1:2,000
Neutralizing/Inhibition 1:100
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

Primarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions.The antiserum does not recognize human IL-1alpha.

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was prepared by repeated immunizations with recombinant human IL-1s produced in E.coli.? The MW of the recombinant 153 aa IL-1s was 17 kDa with the N-terminal amino acid at position alanine 117.? This cleavage site is generated by the IL-1s

Purification

IgG fraction
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_368268

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Cytoplasm, cytosol,Lysosome,Secreted

Background

Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody used in Western Blot (WB). GTX22105

GTX22105 WB Image

GTX22105 was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1beta by Western blot. Lane 1 and 2 were loaded with 2.5 μg and 500 ng of dog IL-1beta respectively.

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX22105

GTX22105 IHC-P Image

IHC-P analysis of medullary lymph node using IL1 beta antibody. Positive staining in the cytoplasm of circulating macrophages. Negative Control (far right) normal rabbit IgG.

REFERENCE

REVIEW

IL1 beta antibody Cat No. GTX22105

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 30987270
Package List Price ($)
$ 379