Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1,000 |
1:100 - 1:200 |
1:400 - 1:800 |
1:500 - 1:2,000 |
1:100 |
Not tested in other applications.
Calculated MW
Product Note
Primarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions.The antiserum does not recognize human IL-1alpha.
Form
Liquid
Buffer
0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1s produced in E.coli.? The MW of the recombinant 153 aa IL-1s was 17 kDa with the N-terminal amino acid at position alanine 117.? This cleavage site is generated by the IL-1s
Purification
IgG fraction
From polyclonal serum
Conjugation
Unconjugated
RRID
AB_368268
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
interleukin 1 beta , IL-1 , IL1-BETA , IL1F2
Cellular Localization
Cytoplasm, cytosol,Lysosome,Secreted
Background
Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.
Database
Research Area