GeneTex
United States (US)

IL24 (Active) recombinant protein

Cat No. GTX65322

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

Measured by its ability to bind to the cell receptor of Capan-1 cells line resulted in Stat-3 activation. The ED50 for this effect is typically 1.0 ng/ml.

Calculated MW

19.5 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

PBS with BSA as a carrier

Storage

Reconstitute in sterile ddH2O. Reconstitute in ddH2O. Store at -20ºC. Product is stable for at least 12 months.

Antigen Species

Human

Expression System

Sacharomyces cerevisiae

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Melanoma differentiationassociated gene 7 protein., IL-24, MDA7, ST16, Suppression of tumorigenicity16 protein, MDA-7, Suppression of tumorigenicity 16 protein, IL24, C49A, FISP, Melanoma differentiation-associated gene 7 protein., Mob5, Mob-5, Melanoma differentiationassociated gene7 protein., IL10B, IL 24, MDA 7, Mob 5

Background

IL24 is a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. IL-10B encoded can induce apoptosis selectively in various cancer cells. Overexpression IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. The glycosylation is essential for activity of IL-24. Functionally, IL-24 has diverse activities. At low concentrations, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1beta, IL-12 and TNF-alpha . At high concentration, it is a strong inducer of apoptosis in tumor cells, but not normal cells. mda-7/IL-24 is being hailed as a ‘magic bullet’ for cancer gene therapy. Recombinant human Il-24 produced in yeast is a single, glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 18 kDa. As a result of glycosilation, the protein migrates at 19.5 kDa on SDS-PAGE.

Research Area

Package List Price ($)
$ 299