Form
Lyophilized powder
Buffer
PBS with BSA as a carrier
Storage
Reconstitute in sterile ddH2O. Reconstitute in ddH2O. Store at -20ºC. Product is stable for at least 12 months.
Antigen Species
Human
Expression System
Sacharomyces cerevisiae
Purification
Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
Melanoma differentiationassociated gene 7 protein., IL-24, MDA7, ST16, Suppression of tumorigenicity16 protein, MDA-7, Suppression of tumorigenicity 16 protein, IL24, C49A, FISP, Melanoma differentiation-associated gene 7 protein., Mob5, Mob-5, Melanoma differentiationassociated gene7 protein., IL10B, IL 24, MDA 7, Mob 5
Background
IL24 is a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. IL-10B encoded can induce apoptosis selectively in various cancer cells. Overexpression IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. The glycosylation is essential for activity of IL-24. Functionally, IL-24 has diverse activities. At low concentrations, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1beta, IL-12 and TNF-alpha . At high concentration, it is a strong inducer of apoptosis in tumor cells, but not normal cells. mda-7/IL-24 is being hailed as a ‘magic bullet’ for cancer gene therapy. Recombinant human Il-24 produced in yeast is a single, glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 18 kDa. As a result of glycosilation, the protein migrates at 19.5 kDa on SDS-PAGE.
Research Area
