APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1 μg/mL |
5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Lymphoid Tissue Tissue Slide(GTX21833) , IL-33 Recombinant Protein(GTX48246-PRO)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Mouse monoclonal IL-33 antibody was raised against the recombinant IL-33 protein.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 33 , C9orf26 , DVS27 , IL1F11 , NF-HEV , NFEHEV
Cellular Localization
Nucleus,Secreted
Background
Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1alphabeta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.
Database
Research Area
DATA IMAGES
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GTX84978 IHC-P Image
IHC-P analysis of human lymph node tissue using GTX84978 IL33 antibody [12H6D12]. Working concentration : 5 μg/ml
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GTX84978 WB Image
WB analysis of 125ng recombinant IL-33 protein using GTX84978 IL33 antibody [12H6D12]. Working concentration : 1 μg/ml
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REFERENCE
There are currently no references for IL33 antibody [12H6D12] (GTX84978). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL33 antibody [12H6D12] (GTX84978). Be the first to share your experience with this product.