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IL33 antibody [12H6D12]

Anti-IL33 antibody [12H6D12] used in IHC (Paraffin sections) (IHC-P). GTX84978
Anti-IL33 antibody [12H6D12] used in Western Blot (WB). GTX84978

Cat No. GTX84978

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 12H6D12

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all IL33 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Human Lymphoid Tissue Tissue Slide(GTX21833) , IL-33 Recombinant Protein(GTX48246-PRO)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Mouse monoclonal IL-33 antibody was raised against the recombinant IL-33 protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 33 , C9orf26 , DVS27 , IL1F11 , NF-HEV , NFEHEV

Cellular Localization

Nucleus,Secreted

Background

Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1alphabeta, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.

Database

Research Area

DATA IMAGES

Anti-IL33 antibody [12H6D12] used in IHC (Paraffin sections) (IHC-P). GTX84978

GTX84978 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX84978 IL33 antibody [12H6D12].
Working concentration : 5 μg/ml

Anti-IL33 antibody [12H6D12] used in Western Blot (WB). GTX84978

GTX84978 WB Image

WB analysis of 125ng recombinant IL-33 protein using GTX84978 IL33 antibody [12H6D12].
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399