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IL6 antibody

Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527

Cat. No. GTX110527

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

recombinant protein IL-6

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human IL6 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721789

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110527

GTX110527 IHC-P Image

IL6 antibody detects IL6 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL6 stained by IL6 antibody (GTX110527) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110527

GTX110527 ICC/IF Image

IL6 antibody detects IL6 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL6 stained by IL6 antibody (GTX110527) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

The data was published in the journal Nat Commun in 2018. PMID: 29422647

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

The data was published in the journal Int J Mol Sci in 2019. PMID: 30987270

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

The data was published in the journal Mol Cancer in 2017.PMID: 28784136

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

The data was published in the journal Stem Cell Res Ther in 2017.PMID: 28482861

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26465338

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

The data was published in the journal Nat Commun in 2018. PMID: 29422647

REFERENCE

REVIEW

IL6 antibody Cat. No. GTX110527

Rating ( Average 4.2 based on 5 users reviews)
Application
Western Blot(WB) ( Average 4 based on 4 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Feb-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Amount used : 10ug
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:500, 16°C, 25Hr
Date : Anonymous submitted on 08-May-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Human lung tumor
Fixation : 4% PFA in PBS, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.25Hr
Primary Antibody : 1:300, 4°C, 12Hr
Figure Note : IHC-P analysis of human lung tumor section using GTX110527 IL6 antibody. Dilution/ 1: 300
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : colon mucosa
Amount used : 25 μg
Blocking : 5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 19-Jul-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : BV2 cells
Amount used : 10 μg
Blocking : Block Ace (DS Parma Biomedical Co. Ltd., Osaka, Japan), 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane 1: Vehicle, Lane 2: LPS (20 ng/ml), Lane 3: LPS (40 ng/ml)
Date : Anonymous submitted on 21-Mar-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20 μg
Blocking : 10% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169