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IL6 antibody

Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110527
Anti-IL6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110527

Cat. No. GTX110527

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

recombinant protein IL-6

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human IL6 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721789

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110527

GTX110527 IHC-P Image

IL6 antibody detects IL6 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL6 stained by IL6 antibody (GTX110527) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110527

GTX110527 ICC/IF Image

IL6 antibody detects IL6 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL6 stained by IL6 antibody (GTX110527) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

IL6 antibody Cat. No. GTX110527

Rating ( Average 4.6 based on 11 users reviews)
Application
Western Blot(WB) ( Average 4.6 based on 10 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Feb-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Amount used : 10ug
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:500, 16°C, 25Hr
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2017.PMID: 28482861
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2017.PMID: 28784136
Date : Anonymous submitted on 08-May-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Human lung tumor
Fixation : 4% PFA in PBS, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.25Hr
Primary Antibody : 1:300, 4°C, 12Hr
Figure Note : IHC-P analysis of human lung tumor section using GTX110527 IL6 antibody. Dilution/ 1: 300
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26465338
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 29422647
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 29422647
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 30987270
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : colon mucosa
Amount used : 25 μg
Blocking : 5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 19-Jul-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : BV2 cells
Amount used : 10 μg
Blocking : Block Ace (DS Parma Biomedical Co. Ltd., Osaka, Japan), 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane 1: Vehicle, Lane 2: LPS (20 ng/ml), Lane 3: LPS (40 ng/ml)
Date : Anonymous submitted on 21-Mar-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20 μg
Blocking : 10% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169