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IL6 antibody

KO/KD Comparison Orthogonal
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110527
Anti-IL6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110527

Cat. No. GTX110527

Reference 35
11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IL6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

recombinant protein IL-6

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human IL6 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721789

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Comparison

IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110527

GTX110527 IHC-P Image

IL6 antibody detects IL6 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL6 stained by IL6 antibody (GTX110527) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110527

GTX110527 ICC/IF Image

IL6 antibody detects IL6 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL6 stained by IL6 antibody (GTX110527) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Hsueh YH et al. J Orthop Translat 2023; 38 : 141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Chiou WC et al. Biomed Pharmacother 2023; 161 : 114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
Application : IHC-P
Reactivity : Mouse
Chini A et al. Sci Rep 2023; 13 (1) : 4036 Novel long non-coding RNAs associated with inflammation and macrophage activation in human.
Application : WB
Reactivity : Human
Mang Y et al. Ann Transl Med 2022; 10 (19) : 1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
Application : WB
Reactivity : Rat
Ko PY et al. Int J Mol Sci 2022; 23 (17) Cross-Linked Hyaluronate and Corticosteroid Combination Ameliorate the Rat Experimental Tendinopathy through Anti-Senescent and -Apoptotic Effects.
Show More +
Submit a Reference

REVIEW

IL6 antibody Cat. No. GTX110527

Rating
( Average 4.6 based on 11 users reviews)
Application
Western Blot(WB)
( Average 4.6 based on 10 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 08-Feb-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Amount used : 10ug
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:500, 16°C, 25Hr
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2017.PMID: 28482861
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mol Cancer in 2017.PMID: 28784136
Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX110527
Date : Anonymous submitted on 08-May-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : Human lung tumor
Fixation : 4% PFA in PBS, 25°C, 0.5Hr
Permeabilization /Antigen retrieval : Citrate buffer, pH 6.0, 0.25Hr
Primary Antibody : 1:300, 4°C, 12Hr
Figure Note : IHC-P analysis of human lung tumor section using GTX110527 IL6 antibody. Dilution/ 1: 300
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26465338
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 29422647
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 29422647
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 30987270
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : colon mucosa
Amount used : 25 μg
Blocking : 5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 19-Jul-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : BV2 cells
Amount used : 10 μg
Blocking : Block Ace (DS Parma Biomedical Co. Ltd., Osaka, Japan), 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane 1: Vehicle, Lane 2: LPS (20 ng/ml), Lane 3: LPS (40 ng/ml)
Anti-IL6 antibody used in Western Blot (WB). GTX110527
Date : Anonymous submitted on 21-Mar-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20 μg
Blocking : 10% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Hsueh YH et al. J Orthop Translat 2023; 38:141-155 Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model.
Chiou WC et al. Biomed Pharmacother 2023; 161:114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
Application : IHC-P
Reactivity : Mouse
Chini A et al. Sci Rep 2023; 13 (1):4036 Novel long non-coding RNAs associated with inflammation and macrophage activation in human.
Application : WB
Reactivity : Human
Liang Y et al. Oxid Med Cell Longev 2022; 2022:3415528 p16(INK4a) Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin.
Application : WB
Reactivity : Mouse
Mang Y et al. Ann Transl Med 2022; 10 (19):1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
Application : WB
Reactivity : Rat
Ko PY et al. Int J Mol Sci 2022; 23 (17) Cross-Linked Hyaluronate and Corticosteroid Combination Ameliorate the Rat Experimental Tendinopathy through Anti-Senescent and -Apoptotic Effects.
Lai KC et al. J Cachexia Sarcopenia Muscle 2022; IFIT2-depleted metastatic oral squamous cell carcinoma cells induce muscle atrophy and cancer cachexia in mice.
Application : WB
Reactivity : Human
Muramatsu R et al. J Pharmacol Sci 2021; 147 (3):294-304 Guanabenz inhibits alveolar bone resorption in a rat model of periodontitis.
Application : IHC-P
Reactivity : Rat
Law YY et al. Aging (Albany NY) 2021; 13 (13):17227-17236 miR-let-7c-5p and miR-149-5p inhibit proinflammatory cytokine production in osteoarthritis and rheumatoid arthritis synovial fibroblasts.
Application : WB
Reactivity : Human
Wang SH et al. J Exp Clin Cancer Res 2021; 40 (1):95 Insulin-like growth factor binding protein 3 promotes radiosensitivity of oral squamous cell carcinoma cells via positive feedback on NF-κB/IL-6/ROS signaling.
Application : IHC-P
Reactivity : Human
Sheu WH et al. Arterioscler Thromb Vasc Biol 2021; 41 (1):e46-e62 Therapeutic Potential of Tpl2 (Tumor Progression Locus 2) Inhibition on Diabetic Vasculopathy Through the Blockage of the Inflammasome Complex.
Application : WB
Reactivity : Mouse
Wang Y et al. Aging (Albany NY) 2020; 12 (20):20184-20197 Interleukin-6 knockout reverses macrophage differentiation imbalance and alleviates cardiac dysfunction in aging mice.
Tsai MH et al. Cells 2020; 9 (8) Ischemic Stroke Risk Associated with Mitochondrial Haplogroup F in the Asian Population.
Application : WB
Reactivity : Human
Liu JF et al. J Cell Physiol 2020; (Epub) PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation.
Application : WB
Reactivity : Human
Liang CC et al. Sci Rep 2020; 10 (1):10030 Effect of amniotic fluid stem cell transplantation on the recovery of bladder dysfunction in spinal cord-injured rats.
Application : IHC-Fr
Reactivity : Rat
Xia Y et al. Neuropsychopharmacology 2020; 45 (11):1896-1908 Modulating microglia activation prevents maternal immune activation induced schizophrenia-relevant behavior phenotypes via arginase 1 in the dentate gyrus.
Application : WB
Reactivity : Mouse
Zhang F et al. Cell Biol Toxicol 2020; (Epub) UCP1 regulates ALDH-positive breast cancer stem cells through releasing the suppression of Snail on FBP1.
Application : WB
Reactivity : Human
Chang JW et al. Cereb Cortex 2020; 30 (8):4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
Application : WB
Reactivity : Rat
Kun-Ming C et al. Arch Med Res 2020; 51 (4):297-302 Potential Anticancer Effect of Celastrol on Hepatocellular Carcinoma by Suppressing CXCR4-related Signal and Impeding Tumor Growth in?Vivo.
Application : WB
Reactivity : Human
Zhou Z et al. J Mol Med (Berl) 2020; (Epub) RIG-I aggravates interstitial fibrosis via c-Myc-mediated fibroblast activation in UUO mice.
Application : WB
Reactivity : Human
Cheng WC et al. Int J Cancer 2019; (Epub) RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.
Application : WB
Reactivity : Human
Lee YC et al. Int J Mol Sci 2019; 20 (7) Essential Role of Visfatin in Lipopolysaccharide and Colon Ascendens Stent Peritonitis-Induced Acute Lung Injury.
Application : WB
Reactivity : Human
Xu X et al. Toxicol Appl Pharmacol 2019; 364:77-82 M2 macrophage-derived IL6 mediates resistance of breast cancer cells to hedgehog inhibition.
Application : WB
Reactivity : Human
D'Eliseo D et al. J Ethnopharmacol 2019; 233:41-46 In vitro studies on anti-inflammatory activities of kiwifruit peel extract in human THP-1 monocytes.
Application : WB
Reactivity : Human
Huang Q et al. J Exp Clin Cancer Res 2018; 37 (1):133 17β-* upregulates il6 expression through the erβ pathway to promote lung adenocarcinoma progression.
Application : IHC-P
Reactivity : Human
Wang Q et al. Nat Commun 2018; 9 (1):559 Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.
Application : WB
Reactivity : Human
Li J et al. Stem Cell Res Ther 2017; 8 (1):107 therapeutic effect of urine-derived stem cells for */lipopolysaccharide-induced interstitial cystitis in a rat model.
Tsai CH et al. Sci Rep 2017; 7 (1):15624 A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of * in prostate cancer.
Application : IHC-P
Reactivity : Human
Kim JY et al. APMIS 2017; 125 (9):808-815 Cytokine expression associated with Helicobacter pylori and Epstein-Barr virus infection in gastric carcinogenesis.
Application : IHC-P
Reactivity : Human
Chang YC et al. Mol Cancer 2017; 16 (1):135 Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.
Application : WB
Reactivity : Human
Liu Q et al. Exp Mol Med 2017; 49 (4):e319 Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.
Application : WB
Reactivity : Human
Kubota K et al. J Otolaryngol Head Neck Surg 2017; 46 (1):36 IL-5 and IL-6 are increased in the frontal recess of eosinophilic chronic rhinosinusitis patients.
Application : IHC-Fr
Reactivity : Human
Dagistan Y et al. J Korean Neurosurg Soc 2017; 60 (1):40-46 Role of Expression of Inflammatory Mediators in Primary and Recurrent Lumbar Disc Herniation.
Application : IHC-P
Reactivity : Human
Liu PL et al. J Agric Food Chem 2015; 63 (43):2768722 Anti-inflammatory Effects of Resveratrol on Hypoxia/Reoxygenation-Induced Alveolar Epithelial Cell Dysfunction.
Application : WB
Reactivity : Human
Tsai CW et al. PLoS One 2015; 10 (10):e0140747 Chitosan Treatment Delays the Induction of Senescence in Human Foreskin Fibroblast Strains.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169