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IL6 antibody

Anti-IL6 antibody used in Western Blot (WB). GTX110527
Anti-IL6 antibody used in Western Blot (WB). GTX110527

Cat No. GTX110527

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

recombinant protein IL-6 , IL6-transfected 293T , *Huh7

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human IL6 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 6,Bsf-2,Bsf2,Cdf,Hgf,Hsf,Ifn-Beta-2,Ifnb2,Il-6,Il6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-IL6 antibody used in Western Blot (WB). GTX110527

GTX110527 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

IL6 antibody Cat No. GTX110527

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Mar-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20 μg
Blocking : 10% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169