WB analysis of recombinant human IL-6 protein using GTX26672 IL6 antibody.
Lane 1 : Protein ladder
Lane 2 : 50 ng
Lane 3 : 20 ng
Lane 4 : 10 ng
Lane 5 : Mock
Dilution : 1:1000

Western blot using GeneTex anti-IL6 antibody (GTX26672).~1 μg of our control protein recombinant human IL-6 was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (GTX27090) secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with chemiluminescent substrate.