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ILK antibody [N1C1-2]

KO/KD
Anti-ILK antibody [N1C1-2] used in Western Blot (WB). GTX107443
Anti-ILK antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX107443
Anti-ILK antibody [N1C1-2] used in Western Blot (WB). GTX107443

Cat. No. GTX107443

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ILK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ILK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950585

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin linked kinase , HEL-S-28 , ILK-1 , ILK-2 , P59 , p59ILK

Cellular Localization

Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ILK antibody [N1C1-2] used in Western Blot (WB). GTX107443

GTX107443 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish heart
10% SDS PAGE
GTX107443 diluted at 1:1000

Anti-ILK antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX107443

GTX107443 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ILK antibody [N1C1-2] (GTX107443) at 1:300 dilution.

Anti-ILK antibody [N1C1-2] used in Western Blot (WB). GTX107443

GTX107443 WB Image

KO/KD

Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1-2] (GTX107443) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169