Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 Âμg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.