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IP6K1 antibody

KO/KD Overexpression
Anti-IP6K1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103949
Anti-IP6K1 antibody used in Western Blot (WB). GTX103949
Anti-IP6K1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103949
Anti-IP6K1 antibody used in Western Blot (WB). GTX103949

Cat. No. GTX103949

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IP6K1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Product Note

Knockdown/Knockout validation was supported by references (PMID:27701146)

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IP6K1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1950559

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inositol hexakisphosphate kinase 1 , IHPK1 , PiUS

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. Alternative splicing occurs for this gene; however, the full-length nature of all transcript variants has not yet been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IP6K1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103949

GTX103949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 Xenograft, using IP6K1(GTX103949) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IP6K1 antibody used in Western Blot (WB). GTX103949

GTX103949 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IP6K1 antibody (GTX103949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IP6K1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103949

GTX103949 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IP6K1(GTX103949) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-IP6K1 antibody used in Western Blot (WB). GTX103949

GTX103949 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IP6K1 antibody (GTX103949) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhu Q et al. Mol Metab 2017; 6 (1) : 73-85 Global IP6K1 deletion enhances temperature modulated energy expenditure which reduces carbohydrate and fat induced weight gain.
Boregowda SV et al. Stem Cells 2017; (Epub) IP6K1 Reduces Mesenchymal Stem/Stromal Cell Fitness and Potentiates High Fat Diet-Induced Skeletal Involution.
Application : WB
Reactivity : Mouse
Zhu Q et al. J Clin Invest 2016; (Epub) Adipocyte-specific deletion of Ip6k1 reduces diet-induced obesity by enhancing AMPK-mediated thermogenesis.
Application : WB
Reactivity : Mouse
Ghoshal S et al. Int J Biochem Cell Biol 2016; 78 : 149-155 Inositol hexakisphosphate kinase-1 interacts with perilipin1 to modulate lipolysis.
Application : IP
Reactivity : Human
Yu W et al. J Biol Chem 2016; Inositol hexakisphosphate kinase 1 (IP6K1) regulates inositol synthesis in mammalian cells.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169