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IP6K1 antibody

KO/KD Overexpression
Anti-IP6K1 antibody used in Western Blot (WB). GTX103949
Anti-IP6K1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103949
Anti-IP6K1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103949
Anti-IP6K1 antibody used in Western Blot (WB). GTX103949

Cat. No. GTX103949

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IP6K1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Product Note

Knockdown/Knockout validation was supported by references (PMID:27701146)

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.22 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IP6K1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1950559

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inositol hexakisphosphate kinase 1 , IHPK1 , PiUS

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. Alternative splicing occurs for this gene; however, the full-length nature of all transcript variants has not yet been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IP6K1 antibody used in Western Blot (WB). GTX103949

GTX103949 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IP6K1 antibody (GTX103949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IP6K1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103949

GTX103949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 Xenograft, using IP6K1(GTX103949) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IP6K1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103949

GTX103949 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IP6K1(GTX103949) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-IP6K1 antibody used in Western Blot (WB). GTX103949

GTX103949 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IP6K1 antibody (GTX103949) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lazcano P et al. J Biol Chem 2022; 298 (9) : 102363 Phosphatidic acid inhibits inositol synthesis by inducing nuclear translocation of kinase IP6K1 and repression of myo-inositol-3-P synthase.
Application : WB, ICC/IF
Reactivity : Mouse
Zhu Q et al. Mol Metab 2017; 6 (1) : 73-85 Global IP6K1 deletion enhances temperature modulated energy expenditure which reduces carbohydrate and fat induced weight gain.
Boregowda SV et al. Stem Cells 2017; (Epub) IP6K1 Reduces Mesenchymal Stem/Stromal Cell Fitness and Potentiates High Fat Diet-Induced Skeletal Involution.
Application : WB
Reactivity : Mouse
Zhu Q et al. J Clin Invest 2016; (Epub) Adipocyte-specific deletion of Ip6k1 reduces diet-induced obesity by enhancing AMPK-mediated thermogenesis.
Application : WB
Reactivity : Mouse
Ghoshal S et al. Int J Biochem Cell Biol 2016; 78 : 149-155 Inositol hexakisphosphate kinase-1 interacts with perilipin1 to modulate lipolysis.
Application : IP
Reactivity : Human
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REVIEW

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Application Reference
Lazcano P et al. J Biol Chem 2022; 298 (9):102363 Phosphatidic acid inhibits inositol synthesis by inducing nuclear translocation of kinase IP6K1 and repression of myo-inositol-3-P synthase.
Application : WB, ICC/IF
Reactivity : Mouse
Zhu Q et al. Mol Metab 2017; 6 (1):73-85 Global IP6K1 deletion enhances temperature modulated energy expenditure which reduces carbohydrate and fat induced weight gain.
Boregowda SV et al. Stem Cells 2017; (Epub) IP6K1 Reduces Mesenchymal Stem/Stromal Cell Fitness and Potentiates High Fat Diet-Induced Skeletal Involution.
Application : WB
Reactivity : Mouse
Zhu Q et al. J Clin Invest 2016; (Epub) Adipocyte-specific deletion of Ip6k1 reduces diet-induced obesity by enhancing AMPK-mediated thermogenesis.
Application : WB
Reactivity : Mouse
Ghoshal S et al. Int J Biochem Cell Biol 2016; 78:149-155 Inositol hexakisphosphate kinase-1 interacts with perilipin1 to modulate lipolysis.
Application : IP
Reactivity : Human
Yu W et al. J Biol Chem 2016; Inositol hexakisphosphate kinase 1 (IP6K1) regulates inositol synthesis in mammalian cells.
Application : WB
Reactivity : Mouse
Rao F et al. Proc Natl Acad Sci U S A 2015; 112 (6):1773-8 Inositol pyrophosphates promote tumor growth and metastasis by antagonizing liver kinase B1.
Application : WB
Reactivity : Human
Rao F et al. Proc Natl Acad Sci U S A 2014; 111 (45):16005-10 Inositol hexakisphosphate kinase-1 mediates assembly/disassembly of the CRL4-signalosome complex to regulate DNA repair and cell death.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169