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IRAK3 antibody

Anti-IRAK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111573
Anti-IRAK3 antibody used in Western Blot (WB). GTX111573

Cat No. GTX111573

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

DDDDK-tagged IRAK3-transfected 293T

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IRAK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 1 receptor associated kinase 3,ASRT5,IRAKM

Background

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-IRAK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111573

GTX111573 ICC/IF Image

IRAK3 antibody detects IRAK3 protein at nucleus by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IRAK3 protein stained by IRAK3 antibody (GTX111573) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-IRAK3 antibody used in Western Blot (WB). GTX111573

GTX111573 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRAK3 antibody (GTX111573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169