WB analysis of heat-shocked COLO205 whole cell lysate using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Dilution : 1:1000

IHC-P analysis of mouse brain tissue using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

ICC/IF analysis of PFA-fixed 3T3 cells using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX05026 IRE1 alpha (phospho Ser724) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/mL

ICC/IF analysis of PFA-fixed HeLa cells using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

IHC-P analysis of human mammary cancer using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of human normal tissue adjacent to colorectal cancer using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of human lung tissue using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of human normal tissue adjacent to lung cancer using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of human pancreatic cancer using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of human normal tissue adjacent to pancreatic cancer using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of rat spleen tissue using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of rat kidney tissue using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

IHC-P analysis of mouse kidney tissue using GTX05026 IRE1 alpha (phospho Ser724) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100