GeneTex
United States (US)

IRF5 antibody [10T1]

GTX39925_4395_WB_18121410_629.jpg
GTX39925_4394_IHC-P_18121410_582.jpg

Cat No. GTX39925

Host Mouse
Clonality Monoclonal
Clone Name 10T1
Isotype IgG1
Application WB, IHC-P, IP, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/1,000-1/2,000
IHC-P 1/50
IP Assay dependent
ELISA Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Not tested in other applications.

Calculated MW

56 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Histidine-tagged recombinant peptide corresponding to amino acids 176-240 of human IRF5, purified from E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interferon Regulatory Factor 5,Sleb10,Irf5

Background

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES
GTX39925_4395_WB_18121410_629.jpg

GTX39925 WB Image

WB analysis of Ramos, THP-1, A20, and NIH3T3 cell lysate using GTX39925 IRF5 antibody [10T1].

GTX39925_4394_IHC-P_18121410_582.jpg

GTX39925 IHC-P Image

IHC-P analysis of human normal colon and colon adenocarcinoma tissue using GTX39925 IRF5 antibody. Heat mediated antigen retrieval using citrate buffer pH6.0.

Package List Price ($)
$ 369