Wild-type (WT) and ISG15 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody (GTX121474) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ISG15 antibody(GTX121474) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ISG15 antibody detects ISG15 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde for 10 min.
Green: ISG15 protein stained by ISG15 antibody (GTX121474) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ISG15 antibody (GTX121474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.