GeneTex
United States (US)

ISG15 antibody

ISG15-antibody__GTX14374-1_18121411_112.jpg
ISG15-antibody-GTX14374-WB-1_18121411_854.jpg

Cat No. GTX14374

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200 - 1:1,000
ELISA 1:2,000 - 1:10,000
IHC Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

Most human cell lysates can be used as a positive control without induction or stimulation
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant human ISG15 protein.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Isg15 Ubiquitin-Like Modifier,G1P2,Ifi15,Imd38,Ip17,Ucrp,Hucrp,Isg15

Cellular Localization

Cytoplasmic and Secreted

Background

ISG15 a 15kDa homologue shows no amino acid sequence homology to cytokines and is synthesized as a precursor that is activated through processing by a thiol protease. ISG15 is secreted by monocytes and lymphocytes. Synthesis is induced in response to IFN alpha or IFN beta, but not IFN gamma. ISG15 expression is induced also by overexpression of some interferon regulatory factors that have been shown to play a role in the transcriptional regulation of IFN genes. ISG15 is secreted also by cell lines of monocyte (U937 cell line), T-lymphocyte, B-lymphocyte (DAUDI cells), human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated, among other things, with alterations in the expression of ISG15. Intracellularly ISG15 has been shown to function as a ubiquitin homologue. It is known also as UCRP (ubiquitin cross-reactive protein). Serpin 2a (spi2a), a member of the serine protease inhibitor (serpin) protein family that is highly induced in macrophages during bacillus Calmette-Guerin infection has been shown to bind ISG15. ISG15 has been shown to modulate immune cell function. It possesses activities of cytokines and induces production of IFN gamma. It enhances proliferation and functions of natural killer and LAK cells.

Database

DATA IMAGES
ISG15-antibody__GTX14374-1_18121411_112.jpg

GTX14374 Image

Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.

ISG15-antibody-GTX14374-WB-1_18121411_854.jpg

GTX14374 WB Image

Western blot analysis ofa GFP-ISG15 fusion proteinwith GTX14374 dilution of the antibody between 1/200 and 1/1000 showed strong reactivity specifically with hISG15 and ISG15 coupled proteins

Package List Price ($)
$ 329