Iba1 antibody [GT10312] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Iba1 antibody [GT10312] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody [GT10312] detects Iba1 protein at microglia by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody [GT10312] (GTX632426) detects AIF1 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: Iba1 antibody [GT10312] (GTX632426) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Iba1 antibody [GT10312] detects Iba1 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody [GT10312] (GTX632426) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [GT10312] (GTX632426) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal Front Mol Neurosci in 2019.PMID: 30914921

The data was published in the journal Front Pharmacol in 2020.PMID: 32194421