Iba1 antibody [HL22]

Name: Iba1 antibody [HL22]
Brand: GeneTex
SKU: GTX635363
Price: 419.00 USD
Rating: 5 (4 reviews)

Cat. No. GTX635363

4 20

Cat. No. GTX635363

GTX635363 IHC-Fr Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

*The competitor is not affiliated with GeneTex and does not endorse this product.

1 / 9

GTX635363 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

2 / 9

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) conjugated with DyLight488 diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

3 / 9

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

4 / 9

GTX635363 ICC/IF Image

Iba1 antibody [HL22] detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:500.
Blue: Hoechst 33342 staining.

5 / 9

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*The competitor is not affiliated with GeneTex and does not endorse this product.

6 / 9

GTX635363 IHC-Fr Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Red: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

7 / 9

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*The competitor is not affiliated with GeneTex and does not endorse this product.

8 / 9

GTX635363 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

9 / 9

This Iba1 antibody [HL22] (GTX635363) is a rabbit monoclonal antibody validated for detection of Iba1 by WB, ICC/IF, IHC-P, IHC-Fr, and IHC (Free Floating). Reactivity has been shown in Human, Mouse, Rat, and other species. This product is cited in 20 published studies.

Host

Rabbit
Clonality

Monoclonal
Clone Name

HL22
Isotype

IgG
Applications

WB ICC/IF IHC-P IHC-Fr IHC (Free Floating)
Reactivity

Human, Mouse, Rat, Pig

Summary

Iba1 antibody detects Iba1, a protein widely used by neuroscientists as a microglial marker and to gauge microglial activation. It is also expressed in macrophages. Discrimination between these two cell populations can be achieved via double staining with TMEM119 antibody (GTX134087), another useful microglia-specific marker, by identifying brain-residing microglia as Iba1+/TMEM119+ while CNS-infiltrating macrophages are Iba1+/TMEM119-.