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Iba1 antibody [HL22]

Recombinant antibody Comparison Orthogonal
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

Cat. No. GTX635363

Reference 10
4

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL22

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Iba1 products
PRODUCT

Summary

Iba1 antibody detects Iba1, a protein widely used by neuroscientists as a microglial marker and to gauge microglial activation. It is also expressed in macrophages. Discrimination between these two cell populations can be achieved via double staining with TMEM119 antibody (GTX134087), another useful microglia-specific marker, by identifying brain-residing microglia as Iba1+/TMEM119+ while CNS-infiltrating macrophages are Iba1+/TMEM119-.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

THP-1 , HL-60

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888516

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363

GTX635363 IHC-Fr Image

Comparison

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

GTX635363 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Comparison

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363

GTX635363 IHC-Fr Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Red: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

GTX635363 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Comparison

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635363

GTX635363 ICC/IF Image

Iba1 antibody [HL22] detects Iba1 protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) conjugated with DyLight488 diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363

GTX635363 IHC-P Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:150.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363

GTX635363 IHC-Fr Image

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Iba1 antibody [HL22] used in Western Blot (WB). GTX635363

GTX635363 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Brown TC et al. bioRxiv 2023; Microglia are dispensable for experience-dependent refinement of visual circuitry.
Wang HK et al. J Inflamm Res 2023; 16 : 4103-4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis.
Wang HK et al. J Inflamm Res 2023; 2023 (16) : 4103—4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis
Application : IHC-Fr
Reactivity : Rat
Iacono D et al. Sci Rep 2023; 13 (1) : 21142 Low-dose brain radiation: lowering hyperphosphorylated-tau without increasing DNA damage or oncogenic activation.
Chia-En Wong et al. J Neuroinflammation 2022; 19 (1) : 153 Sciatic nerve stimulation alleviates acute neuropathic pain via modulation of neuroinflammation and descending pain inhibition in a rodent model.
Show More +
Submit a Reference

REVIEW

Iba1 antibody [HL22] Cat. No. GTX635363

Rating
( Average 5 based on 4 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 3 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Date : Anonymous submitted on 13-Apr-2022
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : human Brain tissue section
Permeabilization /Antigen retrieval : Tris-EDTA pH9.0
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Date : Anonymous submitted on 18-Oct-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : mouse brain
Primary Antibody : 1:150, 4°C, 16Hr
Anti-Iba1 antibody [HL22] used in IHC (Paraffin sections) (IHC-P). GTX635363
Date : Wei-Hao Liao submitted on 21-Sep-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse hippocampus
Fixation : 4% PFA in PBS, 4ºC, overnight
Permeabilization /Antigen retrieval : citric acid (pH6), 25min, 95°C
Primary Antibody : 1:100, 4°C, 16Hr
Anti-Iba1 antibody [HL22] used in IHC (Frozen sections) (IHC-Fr). GTX635363
Date : Anonymous submitted on 16-Jun-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Brain
Blocking : Donkey serum, 25°C, 1Hr
Primary Antibody : 1:300, 4°C, 16Hr
Submit a Review
Application Reference
Brown TC et al. bioRxiv 2023; Microglia are dispensable for experience-dependent refinement of visual circuitry.
Wang HK et al. J Inflamm Res 2023; 16:4103-4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis.
Wang HK et al. J Inflamm Res 2023; 2023 (16):4103—4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis
Application : IHC-Fr
Reactivity : Rat
Iacono D et al. Sci Rep 2023; 13 (1):21142 Low-dose brain radiation: lowering hyperphosphorylated-tau without increasing DNA damage or oncogenic activation.
Chia-En Wong et al. J Neuroinflammation 2022; 19 (1):153 Sciatic nerve stimulation alleviates acute neuropathic pain via modulation of neuroinflammation and descending pain inhibition in a rodent model.
Fan Z et al. Ecotoxicol Environ Saf 2022; 234:113372 JAK2/STAT3 pathway regulates microglia polarization involved in hippocampal inflammatory damage due to acute paraquat exposure.
Application : WB
Reactivity : Rat
Zhang Y et al. Cell Death Dis 2021; 12 (12):1159 TRPV1 channel mediates NLRP3 inflammasome-dependent neuroinflammation in microglia.
Application : IHC-P
Reactivity : Mouse
Yan H et al. Neuropharmacology 2021; 194:108627 Parecoxib alleviates the motor behavioral decline of aged rats by ameliorating mitochondrial dysfunction in the substantia nigra via COX-2/PGE2 pathway inhibition.
Application : IHC-P
Reactivity : Rat
Xiong LL et al. Am J Chin Med 2021; 49 (3):677-703 Effect of Sutellarin on Neurogenesis in Neonatal Hypoxia-Ischemia Rat Model: Potential Mechanisms of Action.
Teng SW et al. Cell Biol Int 2021; 45 (5):1072-1081 Potential surrogate quantitative immunomodulatory potency assay for monitoring human umbilical cord-derived mesenchymal stem cells production.
Application : IHC-P
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations