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Influenza A virus M1 (matrix protein) antibody

Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX127356

Cat No. GTX127356

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Influenza A virus
Package
100 μl ($329),
25 μl ($169)
See all Influenza A virus M1 (matrix protein) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

DF-1 , WSN virus infected DF-1

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the Internal region of Influenza A virus M1 (matrix protein) (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885647

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Influenza A Virus Membrane 1 protein , Influenza A Virus M1

Background

Influenza A virus matrix 1, M1, is a critical protein required for assembly and budding. Hemagglutinin associates with M1 via its cytoplasmic tail and transmembrane domain. The M2 and nucleoprotein proteins are critical in the replication cycle of influenza viruses. The M2 channel protein is an essential component of the viral envelope because of its ability to form a highly selective, pH-regulated, proton-conducting channel. The M2 channel allows protons to enter the interior of the virus and acidification weakens the interaction of the M1 protein with the ribonuclear core.

Research Area

DATA IMAGES

Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX127356

GTX127356 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus M1 (matrix protein) antibody (GTX127356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Russier M et al. J Virol 2020; 94 (3) Hemagglutinin Stability Regulates H1N1 Influenza Virus Replication and Pathogenicity in Mice by Modulating Type I Interferon Responses in Dendritic Cells.
Marion Russier et al. bioRxiv 2019; (Epub) HA stability regulates H1N1 influenza virus replication and pathogenicity in mice by modulating type I interferon responses in dendritic cells.
Application : WB
Deo VK et al. Mol Pharm 2015; 12 (3) : 839-45 Chimeric virus-like particles made using GAG and * capsid proteins providing dual drug delivery and vaccination platform.
Application : ELISA
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169