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Influenza A virus M1 (matrix protein) antibody

Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX127356

Cat No. GTX127356

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Influenza A virus
Package
100 μl ($319),
25 μl ($169)
See all Influenza A virus Matrix protein products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

DF-1 , WSN virus infected DF-1
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus ( H1N1)

Immunogen

Recombinant fragment corresponding to Internal region of Influenza A virus M1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Influenza A virus matrix 1, M1, is a critical protein required for assembly and budding. Hemagglutinin associates with M1 via its cytoplasmic tail and transmembrane domain. The M2 and nucleoprotein proteins are critical in the replication cycle of influenza viruses. The M2 channel protein is an essential component of the viral envelope because of its ability to form a highly selective, pH-regulated, proton-conducting channel. The M2 channel allows protons to enter the interior of the virus and acidification weakens the interaction of the M1 protein with the ribonuclear core.

Research Area

DATA IMAGES
Anti-Influenza A virus M1 (matrix protein) antibody used in Western Blot (WB). GTX127356

GTX127356 WB Image

Influenza A Virus M1 antibody detects Influenza A Virus M1 protein by Western blot analysis.
A. 5 ug DF-1 whole cell lysate/extract
B. 5 ug whole cell lysate/extract WSN virus infected DF-1 cells
12 % SDS-PAGE
Influenza A Virus M1 antibody (GTX127356) dilution: 1:1000
REFERENCE

Application Reference

Deo VK et al. Mol Pharm 2015; 12 (3) : 839-45 Chimeric virus-like particles made using GAG and * capsid proteins providing dual drug delivery and vaccination platform.
Application : ELISA
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169