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Influenza A virus Nucleoprotein antibody [HL1089]

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247
Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

Cat. No. GTX636247

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1089

Isotype

IgG

Application

WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

Influenza A virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Lateral Flow
Capture : GTX637790, Detection : GTX636247 or Capture : GTX636247 / GTX636318, Detection : GTX636199 or Capture : GTX636247 / GTX636199, Detection : GTX636318. Please notice that GTX636247 needs to be conjugated to Gold to function as the detection antibody when paired with GTX637790. Please contact us for custom Gold-conjugated antibody.

Sandwich ELISA
Capture : GTX636318 / GTX636247, Detection : GTX636247 / GTX636318. Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318 / GTX637790. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody is specific for Influenza A virus Nucleoprotein protein (H1N1, H3N2, H5N8, and H10N3), and it does not cross-react with Influenza B virus Nucleoprotein protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza A virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Influenza A virus Nucleoprotein (A/Kansas/14/2017(H3N2)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909956

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247

GTX636247 Lateral Flow Image

Detection of recombinant influenza A virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Capture: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
Detection: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Indirect ELISA analysis was performed by coating a plate with recombinant influenza A virus nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag (GTX135903-pro) (50 ng), and probing with the specified influenza A virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza A virus or influenza B virus and probing with the specified influenza A virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636247

GTX636247 ICC/IF Image

Influenza A virus Nucleoprotein antibody (GTX636247) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX636247) diluted at 1:2000.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

GTX636247 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus NP (nucleoprotein) antibody [HL1078] (GTX636199) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/Singapore/63/04 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below.
Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 μg/mL)

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below.
Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 μg/mL)

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1); A/Singapore/63/04 (H1N1); A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) (1-0.2 μg). Coated protein was probed with Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636247

GTX636247 ICC/IF Image

Immunofluorescent analysis of influenza virus infected cells using Influenza A virus Nucleoprotein antibody (GTX636247).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody (GTX636247) diluted at 1:100.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 μg/mL).

Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636247. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza A virus NP (nucleoprotein) antibody (GTX135903-pro) (50 ng). Coated protein was probed with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

EC50 : 37.93 pM

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

GTX636247 WB Image

Influenza A viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in ELISA (ELISA). GTX636247

GTX636247 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987)) using antibodies as below.
Capture : Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (5 μg/mL)
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (1 μg/mL).

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636247

GTX636247 IHC-P (cell pellet) Image

Influenza A virus Nucleoprotein antibody [HL1089] detects Influenza A virus Nucleoprotein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded flu A_NP (Kansas/2017/H3N2) transfected 293T cell FFPE Cell Pellet Block.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody [HL1089] (GTX636318) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247

GTX636247 Lateral Flow Image

Detection of influenza A virus nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Influenza A virus Nucleoprotein antibody (GTX636247 [HL1089])
Detection: Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])

Samples (1 ng) :
1. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
2. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
3. Lysis buffer

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Lateral Flow (Lateral Flow). GTX636247

GTX636247 Lateral Flow Image

Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

GTX636247 WB Image

Influenza A viral lysate (0.1 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Influenza A virus Nucleoprotein antibody [HL1089] used in Western Blot (WB). GTX636247

GTX636247 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Influenza A virus Nucleoprotein antibody [HL1089] Cat. No. GTX636247

Rating ( Average 5 based on 2 users reviews)
Application
Lateral Flow(Lateral Flow) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 28-Sep-2021
Score :
Application Tested : Lateral Flow
Figure Note : Detection of recombinant influenza A virus nucleoproteins of the indicated strains by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

Capture: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
Detection: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Date : Anonymous submitted on 03-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : Influenza A virus
Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)
Figure Note : Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Package List Price ($)
$ 399
$ 169
Custom Conjugations