Influenza B virus Nucleoprotein antibody [HL1073]

Name: Influenza B virus Nucleoprotein antibody [HL1073]
Brand: GeneTex
SKU: GTX636194
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Influenza B virus Nucleoprotein antibody [HL1073] used in ELISA (ELISA). GTX636194

Anti-Influenza B virus Nucleoprotein antibody [HL1073] used in Lateral Flow (Lateral Flow). GTX636194

Anti-Influenza B virus Nucleoprotein antibody [HL1073] used in Western Blot (WB). GTX636194

Anti-Influenza B virus Nucleoprotein antibody [HL1073] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636194

Cat. No. GTX636194

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1073
Isotype	IgG
Application	WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA
Reactivity	Influenza B virus
Package	100 μl ($399), 25 μl ($169)

See all Influenza B virus Nucleoprotein products

Datasheet

View product citations for antibody GTX636194 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
Note : Lateral Flow Capture : GTX636194, Detection : GTX636099 / GTX636100 Please notice that the detection antibodies need to be conjugated to Gold to function when paired with capture antibodies. Please contact us for custom Gold-conjugated antibody. Sandwich ELISA Capture : GTX636194, Detection : GTX636099 / GTX636100 or Capture : GTX636099 / GTX636100, Detection : GTX636194. Please notice GTX63 6194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099 / GTX636100.. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Influenza B virus

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909950

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636194 ELISA Image

Indirect ELISA analysis was performed by coating a plate with recombinant influenza B virus nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) (50 ng), and probing with the specified influenza B virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX636194 ELISA Image

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX636194 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (20000-4.77*10^-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 29.94 pM

GTX636194 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

GTX636194 Lateral Flow Image

Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Sample : Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)

GTX636194 ELISA Image

Sandwich ELISA detection of recombinant Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL)

Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

GTX636194 WB Image

Influenza B viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636194 ELISA Image

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

GTX636194 ELISA Image

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 μg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX636194 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

GTX636194 ICC/IF Image

Immunofluorescent analysis of influenza virus infected cells using Influenza B virus Nucleoprotein antibody (GTX636194).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza B virus Nucleoprotein antibody (GTX636194) diluted at 1:100.

GTX636194 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL).

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

GTX636194 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL)

Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

GTX636194 ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below.
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL)
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL).

Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

GTX636194 ICC/IF Image

Influenza B virus Nucleoprotein antibody (GTX636100) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX636100) diluted at 1:2000.

GTX636194 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

GTX636194 Lateral Flow Image

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.

Capture:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635688 [HL455])
A. Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])
B. Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])
Detection:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635686 [HL448])
A. Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])
B. Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])

Samples (40 ng) :
1. Lysis buffer
2. SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag (GTX135592-pro)
3. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
4. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
5. Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)
6. Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag (GTX136277-pro)
7. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

GTX636194 Lateral Flow Image

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.
Pair for S :
Capture : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL455] (GTX635688)
Detection : SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511] (GTX635689)
Pair for A:
Capture : Influenza A virus Nucleoprotein antibody [HL1952] (GTX637789)
Detection : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
Pair for B :
Capture : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)
Detection : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)

Samples (40 ng) :
1. Lysis buffer
2. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

GTX636194 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636194 Lateral Flow Image

Detection of influenza B virus nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])
Detection: Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])

Samples (2.5 ng) :
1. Influenza B virus Nucleoprotein (B/Sydney/3/2004) (GTX135867-pro)
2. Influenza B virus Nucleoprotein (B/Victoria/02/1987) (GTX136277-pro)
3. Lysis buffer

REFERENCE

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REVIEW

Influenza B virus Nucleoprotein antibody [HL1073] Cat. No. GTX636194

Rating

( Average 5 based on 1 users reviews)

Application

Lateral Flow(Lateral Flow)

( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 03-Sep-2021

Score :

Application Tested : Lateral Flow

Sample Species : Influenza B virus

Sample : Influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)

Figure Note : Detection of recombinant influenza B virus nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1068 : Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099)
HL1069 : Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100)
HL1073 : Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194)

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Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty Custom Conjugations

Influenza B virus Nucleoprotein antibody [HL1073]

Cat. No. GTX636194

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Research Area

DATA IMAGES

GTX636194 ELISA Image

GTX636194 ELISA Image

GTX636194 ELISA Image

GTX636194 ELISA Image

GTX636194 ELISA Image

GTX636194 Lateral Flow Image

GTX636194 ELISA Image

GTX636194 WB Image

GTX636194 ELISA Image

GTX636194 ELISA Image

GTX636194 ELISA Image

GTX636194 ICC/IF Image

GTX636194 ELISA Image

GTX636194 ELISA Image

GTX636194 ELISA Image

GTX636194 ICC/IF Image

GTX636194 ELISA Image

GTX636194 Lateral Flow Image

GTX636194 Lateral Flow Image

GTX636194 WB Image

GTX636194 Lateral Flow Image

REFERENCE

REVIEW

Influenza B virus Nucleoprotein antibody [HL1073] Cat. No. GTX636194

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.