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Integrin beta 1 / CD29 antibody [12G10]

Anti-Integrin beta 1 / CD29 antibody [12G10] used in Flow cytometry (FACS). GTX75707

Cat No. GTX75707

Host Mouse
Clonality Monoclonal
Clone Name 12G10
Isotype IgG1
Application WB, ICC/IF, IHC-Fr, FACS, IP, ELISA
Reactivity Human, Mink
Package
200 μg ($349)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1/25-1/50
IP Assay dependent
ELISA 10μg/ml

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

88 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Integrin Subunit Beta 1,Cd29,Fnrb,Gpiia,Mdf2,Msk12,Vla-Beta,Vlab,Itgb1

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Integrin beta 1 / CD29 antibody [12G10] used in Flow cytometry (FACS). GTX75707

GTX75707 FACS Image

FACS analysis of human peripheral blood monocytes using GTX75707 Integrin beta 1 / CD29 antibody [12G10].

REFERENCE
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REVIEW
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Package List Price ($)
$ 349