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Integrin beta 1 / CD29 antibody

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Immunoprecipitation (IP). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

Cat. No. GTX128839

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885823

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane,Cleavage furrow,Melanosome

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Wild-type (WT) and Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:400. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Wild-type (WT) and Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:400. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000 and competitor's antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128839

GTX128839 ICC/IF Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Integrin beta 1 / CD29 antibody used in Immunoprecipitation (IP). GTX128839

GTX128839 IP Image

Immunoprecipitation of Integrin beta 1 / CD29 protein from HeLa whole cell extracts using 5 μg of Integrin beta 1 / CD29 antibody (GTX128839).
Western blot analysis was performed using Integrin beta 1 / CD29 antibody (GTX128839).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128839

GTX128839 ICC/IF Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

The data was published in the journal Oncotarget in 2017. PMID: 29050309

REFERENCE

REVIEW

Integrin beta 1 / CD29 antibody Cat. No. GTX128839

Rating ( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 17-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : 14dpp C57BL/6J mouse testis
Blocking : 5% donkey-serum in TNB, 25°C, 1Hr
Fixation : methanol:acetone = 1:1 for 7min
Primary Antibody :
Figure Note : Peritubular myoid cells (PMCs, arrows; smooth muscle actin (SMA) is a marker) which surround seminiferous tubules (*) express integrin beta 1.
Red : Integrin beta 1 / CD29
Green : SMA
Blue : DAPI
Date : Anonymous submitted on 18-Jun-2019
Score :
Application Tested : WB
Sample Species : Hu, Ms
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 25°C, 1Hr
Lane Description : Lane 1: mouse endothelial cells; Lane 2: TOV112D; Lane 3: CaCo2; Lane 4: HT29
Date : Anonymous submitted on 13-Dec-2013
Score :
Application Tested : IP
Sample Species : Hu
Sample : J82 cells
Amount used : 500μg
Blocking : 5% BSA, 25°C, 2Hr
Fixation : NP-40 lysis buffer
Primary Antibody : 1:10000, 4°C, 16Hr
Figure Note : 1 ug for IP
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169