GeneTex
  • Country / Location Selection

United States (US)

Integrin beta 1 / CD29 antibody

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Immunoprecipitation (IP). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839
Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128839

Cat No. GTX128839

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat, Dog
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

A431 , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2 , *J82

Predict Reactivity

Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Integrin beta 1 / CD29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885823

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB

Cellular Localization

Cell membrane,Cleavage furrow,Melanosome

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Wild-type (WT) and Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000 and competitor's antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Integrin beta 1 / CD29 antibody used in Immunoprecipitation (IP). GTX128839

GTX128839 IP Image

Immunoprecipitation of Integrin beta 1 / CD29 protein from HeLa whole cell extracts using 5 μg of Integrin beta 1 / CD29 antibody (GTX128839).
Western blot analysis was performed using Integrin beta 1 / CD29 antibody (GTX128839).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Integrin beta 1 / CD29 antibody used in IHC (Paraffin sections) (IHC-P). GTX128839

GTX128839 IHC-P Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128839

GTX128839 ICC/IF Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Integrin beta 1 / CD29 antibody used in Western Blot (WB). GTX128839

GTX128839 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Integrin beta 1 / CD29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128839

GTX128839 ICC/IF Image

Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

Integrin beta 1 / CD29 antibody Cat No. GTX128839

Rating ( Average 4.5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 17-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : 14dpp C57BL/6J mouse testis
Blocking : 5% donkey-serum in TNB, 25°C, 1Hr
Fixation : methanol:acetone = 1:1 for 7min
Primary Antibody :
Figure Note : Peritubular myoid cells (PMCs, arrows; smooth muscle actin (SMA) is a marker) which surround seminiferous tubules (*) express integrin beta 1.
Red : Integrin beta 1 / CD29
Green : SMA
Blue : DAPI
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017.PMID: 29050309
Date : Anonymous submitted on 18-Jun-2019
Score :
Application Tested : WB
Sample Species : Hu, Ms
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 25°C, 1Hr
Lane Description : Lane 1: mouse endothelial cells; Lane 2: TOV112D; Lane 3: CaCo2; Lane 4: HT29
Date : Anonymous submitted on 13-Dec-2013
Score :
Application Tested : IP
Sample Species : Hu
Sample : J82 cells
Amount used : 500μg
Blocking : 5% BSA, 25°C, 2Hr
Fixation : NP-40 lysis buffer
Primary Antibody : 1:10000, 4°C, 16Hr
Figure Note : 1 ug for IP
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf