APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| Assay dependent |
| 1:100-1:1000 |
| Assay dependent |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat-2
Predict Reactivity
Sheep, Bovine, Cat, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.04 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Integrin beta 1 / CD29. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885823
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
integrin subunit beta 1 , CD29 , FNRB , GPIIA , MDF2 , MSK12 , VLA-BETA , VLAB
Cellular Localization
Cell membrane,Cleavage furrow,Melanosome
Background
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX128839 WB Image
Wild-type (WT) and Integrin beta 1 / CD29 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:400. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000 and competitor's antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX128839 WB Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg BCL-1 whole cell lysate/extract
C. 30 μg Raw264.7 whole cell lysate/extract
D. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein on breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Integrin beta 1 / CD29 antibody (GTX128839) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX128839 IP Image
Immunoprecipitation of Integrin beta 1 / CD29 protein from HeLa whole cell extracts using 5 μg of Integrin beta 1 / CD29 antibody (GTX128839).
Western blot analysis was performed using Integrin beta 1 / CD29 antibody (GTX128839).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX128839 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128839 ICC/IF Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX128839 WB Image
Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)
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GTX128839 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 ICC/IF Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX128839 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brown adipocyte.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX128839 IHC-P Image
Integrin beta 1 / CD29 antibody detects Integrin beta 1 / CD29 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Integrin beta 1 / CD29 stained by Integrin beta 1 / CD29 antibody (GTX128839) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
Integrin beta 1 / CD29 antibody
Cat. No. GTX128839
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Rating
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( Average 4.5 based on 4 users reviews)
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Application
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Western Blot(WB)
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( Average 4.5 based on 2 users reviews)
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IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 1 users reviews)
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Immunoprecipitation(IP)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 17-Aug-2021
Application Tested :
IHC-Fr
Sample Species :
Ms
Sample :
14dpp C57BL/6J mouse testis
Blocking :
5% donkey-serum in TNB, 25°C, 1Hr
Fixation :
methanol:acetone = 1:1 for 7min
Primary Antibody :
Figure Note :
Peritubular myoid cells (PMCs, arrows; smooth muscle actin (SMA) is a marker) which surround seminiferous tubules (*) express integrin beta 1.
Red : Integrin beta 1 / CD29
Green : SMA
Blue : DAPI
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2017.
PMID: 29050309
Date :
Anonymous submitted on 18-Jun-2019
Application Tested :
WB
Sample Species :
Hu, Ms
Amount used :
30 μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:500, 25°C, 1Hr
Lane Description :
Lane 1: mouse endothelial cells; Lane 2: TOV112D; Lane 3: CaCo2; Lane 4: HT29
Date :
Anonymous submitted on 13-Dec-2013
Application Tested :
IP
Sample Species :
Hu
Sample :
J82 cells
Amount used :
500μg
Blocking :
5% BSA, 25°C, 2Hr
Fixation :
NP-40 lysis buffer
Primary Antibody :
1:10000, 4°C, 16Hr
Figure Note :
1 ug for IP
