GeneTex
United States (US)

Interferon gamma Receptor 1 antibody

Cat No. GTX10745

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA, Neut
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 μg/ml
ICC/IF 5-15 μg/ml
ELISA Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

human peripheral blood mononuclear cells
PROPERTIES

Form

Liquid

Buffer

PBS containing carbohydrates

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

recombinant human IFN-γ Receptor I extracellular domain expressed in NSO cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interferon Gamma Receptor 1,Cd119,Ifngr,Imd27A,Imd27B,Ifngr1

Background

The IFNG receptor complex is composed of two type I membrane proteins, IFNGRI (IFNGR alpha) and IFNGR2 (IFNGR beta). Both receptor proteins are members of the type II cytokine receptor family and share 52% overall sequence identity. The IFNG receptor is constitutively expressed in most cell types. Soluble IFNG has been detected in biological fluids. IFNG R1 is required for ligand-binding and signaling. It binds to IFNG with high affinity and is a potent IFNG antagonist. In signal transduction, IFNG induces tyrosine phosphorylation of IFNG R1 leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFNg-induced gene transcription. Human IFNGRI, a 227 amino acid residue protein, has a calculated molecular mass of approximately 25 kDa. As a result of glycosylation, the recombinant IFNGRI migrates as a 40 to 50 kDa protein in SDS-PAGE. Human and mouse IFNGRI share approximately 52% amino acid sequence identity and each interacts with IFNG in a strictly species-specific manner.

Database

Research Area