GeneTex
United States (US)

Jurkat whole cell lysate

Jurkat-whole-cell-lysate_WB_GTX30596-1_18121411_682.jpg

Cat No. GTX30596

Application WB
Species Human
PRODUCT

Product Description

Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na₃VO₄ were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.
APPLICATION

Positive Control

Western analysis positive control. Note that Jurkat is only applicable as a positive control for specific antibodies.
PROPERTIES

Form

Liquid

Buffer

1 x SDS sample buffer containing 5% β-mercaptoethanol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
Jurkat-whole-cell-lysate_WB_GTX30596-1_18121411_682.jpg

GTX30596 WB Image

Coommassie stained SDS-PAGE of 20 µg of (1) Hela WCL, (2) Jurkat WCL (GTX30596), (3) HEK 293 WCL, (4) MCF-7 WCL, (5) A 549 WCL, (6) Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.

Package List Price ($)
$ 179