Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1536] (GTX637010) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KAP1 antibody [HL1536] detects KAP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KAP1 stained by KAP1 antibody [HL1536] (GTX637010) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.