APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tripartite motif containing 28 , KAP1 , PPP1R157 , RNF96 , TF1B , TIF1B
Cellular Localization
Nucleus
Background
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-KAP1 antibody [HL1536] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637010](/templates/default/js/lightbox/images/loading.gif "Anti-KAP1 antibody [HL1536] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637010")
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GTX637010 ICC/IF Image
KAP1 antibody [HL1536] detects KAP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: KAP1 stained by KAP1 antibody [HL1536] (GTX637010) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX637010 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1536] (GTX637010) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for KAP1 antibody [HL1536] (GTX637010). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for KAP1 antibody [HL1536] (GTX637010). Be the first to share your experience with this product.
![Anti-KAP1 antibody [HL1536] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637010](/upload/website/prouct_img/small/GTX637010/GTX637010_T-44690_20220701_ICC_IF_22071401_907.webp "Anti-KAP1 antibody [HL1536] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637010")
![Anti-KAP1 antibody [HL1536] used in Western Blot (WB). GTX637010](/upload/website/prouct_img/small/GTX637010/GTX637010_44739_20220715_WB_22072519_502.webp "Anti-KAP1 antibody [HL1536] used in Western Blot (WB). GTX637010")
![Anti-KAP1 antibody [HL1536] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637010](/upload/website/prouct_img/normal/GTX637010/GTX637010_T-44690_20220701_ICC_IF_22071401_907.webp "Anti-KAP1 antibody [HL1536] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637010")
![Anti-KAP1 antibody [HL1536] used in Western Blot (WB). GTX637010](/upload/website/prouct_img/normal/GTX637010/GTX637010_44739_20220715_WB_22072519_502.webp "Anti-KAP1 antibody [HL1536] used in Western Blot (WB). GTX637010")