Western blot – Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Reacts with residues 26-36 [ERKRHDSERST] of the FKLF protein. Tested in human fetal liver tissue and the K562 erythroid cell line. Low levels of protein seen in other adult and fetal tissues or non-erythroid cell lines.
PBS containing 0.02% sodium azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Synthetic peptide: ERKRHDSERST, corresponding to amino acids 26-36 of Human FKLF.
Affinity purified IgG
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TIEG2, 603301, FKLF1, KLF11, 8462, FKLF, O14901, MODY7, Tieg3
A novel Kruppel-type zinc finger protein, FKLF, that was cloned from globin-expressing human fetal erythroid cells. FKLF acts as a transcriptional activator of embryonic and fetal beta-globin genes.