GeneTex
United States (US)

KLF5 antibody

KO/KD Orthogonal Overexpression
GTX103289_42872_20190322_WB_KO_watermark_19040219_913.jpg
GTX103289_42872_20170721_WB_CoCl2_MG132_19011506_998.jpg
GTX103289_42872_20170811_WB_19011506_249.jpg
GTX103289_42914_20170906_IFA_19011506_966.jpg
GTX103289_39876_20170504_WB_B_19011506_499.jpg
GTX103289_42872_20170622_WB_19011506_430.jpg
GTX103289_39876_IHC_19011506_439.jpg

Cat No. GTX103289

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

A549 , HCT116 , KLF5-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kruppel Like Factor 5,Bteb2,Cklf,Iklf,Klf5

Cellular Localization

Nucleus

Background

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103289_42872_20190322_WB_KO_watermark_19040219_913.jpg

GTX103289 WB Image

KO/KD

Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103289_42872_20170721_WB_CoCl2_MG132_19011506_998.jpg

GTX103289 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103289_42872_20170811_WB_19011506_249.jpg

GTX103289 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103289_42914_20170906_IFA_19011506_966.jpg

GTX103289 ICC/IF Image

KLF5 antibody detects KLF5 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KLF5 protein stained by KLF5 antibody (GTX103289) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

GTX103289_39876_20170504_WB_B_19011506_499.jpg

GTX103289 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103289_42872_20170622_WB_19011506_430.jpg

GTX103289 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103289_39876_IHC_19011506_439.jpg

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
KLF5 antibody (GTX103289) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

KLF5 antibody Cat No. GTX103289

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse heart tissue
Blocking : 3%BSA in PBST, 25°C, 1Hr
Fixation : 4%PFA in PBS
Primary Antibody : 1:1500, 4°C, 15Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf