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KLF5 antibody

KO/KD Orthogonal Overexpression
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

Cat. No. GTX103289

Reference 23
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all KLF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

KLF5-transfected 293T , HeLa (500 μM CoCl2 treatment for 24 hr and 10 μM MG132 treatment for 2 hr)

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950625

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Kruppel like factor 5 , BTEB2 , CKLF , IKLF

Cellular Localization

Nucleus

Background

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

KO/KD

Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
KLF5 antibody (GTX103289) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103289

GTX103289 ICC/IF Image

KLF5 antibody detects KLF5 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

KO/KD Orthogonal Overexpression

The data was published in the journal Mediators Inflamm in 2014. PMID: 25197166

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Orthogonal

The data was published in the journal Biosci Rep in 2014. PMID: 25205373

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Orthogonal

The data was published in the journal Mediators Inflamm in 2014. PMID: 25197166

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Orthogonal

The data was published in the journal Biosci Rep in 2014. PMID: 25205373

Anti-KLF5 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX103289

GTX103289 WB IP Image

Orthogonal

The data was published in the journal Mediators Inflamm in 2014. PMID: 25197166

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Orthogonal

The data was published in the journal Mediators Inflamm in 2014. PMID: 25197166

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Orthogonal

The data was published in the journal Mediators Inflamm in 2014. PMID: 25197166

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

KO/KD Overexpression

The data was published in the journal Biosci Rep in 2014. PMID: 25205373

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

The data was published in the journal Mediators Inflamm in 2014. PMID: 25197166

REFERENCE

Application Reference

Zhang X et al. Redox Biol 2024; 75 : 103246 Lactate drives epithelial-mesenchymal transition in diabetic kidney disease via the H3K14la/KLF5 pathway.
Derk J et al. Dev Cell 2023; 58 (8) : 635-644.e4 Formation and function of the meningeal arachnoid barrier around the developing mouse brain.
V Pl? et al. Fluids Barriers CNS 2023; 20 (1) : 93 Structural characterization of SLYM - a 4th cranial meningeal membrane
Dong Ma et al. BMC Cancer 2021; 21 (1) : 377 KLF5-mediated Eppk1 expression promotes cell proliferation in cervical cancer via the p38 signaling pathway.
Application : WB, IHC-P
Reactivity : Human
Annam?ria Nagy et al. Int J Mol Sci 2021; 22 (17) BGP-15 Inhibits Hyperglycemia-Aggravated VSMC Calcification Induced by High Phosphate.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

KLF5 antibody Cat. No. GTX103289

Rating
( Average 3 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 3 based on 1 users reviews)
Anti-KLF5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX103289
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse heart tissue
Blocking : 3%BSA in PBST, 25°C, 1Hr
Fixation : 4%PFA in PBS
Primary Antibody : 1:1500, 4°C, 15Hr
Submit a Review
Application Reference
Zhang X et al. Redox Biol 2024; 75:103246 Lactate drives epithelial-mesenchymal transition in diabetic kidney disease via the H3K14la/KLF5 pathway.
Derk J et al. Dev Cell 2023; 58 (8):635-644.e4 Formation and function of the meningeal arachnoid barrier around the developing mouse brain.
V Pl? et al. Fluids Barriers CNS 2023; 20 (1):93 Structural characterization of SLYM - a 4th cranial meningeal membrane
Dong Ma et al. BMC Cancer 2021; 21 (1):377 KLF5-mediated Eppk1 expression promotes cell proliferation in cervical cancer via the p38 signaling pathway.
Application : WB, IHC-P
Reactivity : Human
Annam?ria Nagy et al. Int J Mol Sci 2021; 22 (17) BGP-15 Inhibits Hyperglycemia-Aggravated VSMC Calcification Induced by High Phosphate.
Application : WB
Reactivity : Human
Dong Ma et al. Front Cardiovasc Med 2020; 7:592550 Vascular Smooth Muscle FTO Promotes Aortic Dissecting Aneurysms via m6A Modification of Klf5.
Application : WB
Reactivity : Human
Ma D et al. PLoS Biol 2020; 18 (8):e3000808 Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.
Application : WB
Reactivity : Human
Sun Y et al. Cell Death Dis 2020; 11 (6):432 Increased AT2R expression is induced by AT1R autoantibody via two axes, Klf-5/IRF-1 and circErbB4/miR-29a-5p, to promote VSMC migration.
Application : WB, IHC-P
Reactivity : Mouse
Dong Ma et al. bioRxiv 2019; (Epub) eIF5a reduction via decreased Klf5 leads to cell senescence by mitochondrial fission in VSMCs.
Application : WB
Reactivity : Mouse
Xue-shan Zhao et al. Phytomedicine 2018; (Epub) Salvianolic acid B inhibits Ang II-induced VSMC proliferation in vitro and intimal hyperplasia in vivo by downregulating miR-146a expression.
Application : WB
Reactivity : Mouse
Marrero-Rodriguez D et al. Arch Med Res 2017; 48 (4):314-322 Kruppel Like Factors Family Expression in Cervical Cancer Cells.
Application : ICC/IF
Reactivity : Human
Ma D et al. Sci Rep 2017; 7 (1):15683 KLF5 promotes cervical cancer proliferation, migration and invasion in a manner partly dependent on TNFRSF11a expression.
Application : IHC-P
Reactivity : Human
Miura S et al. Cell Stem Cell 2017; 21 (4):456-471.e5 Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming.
Application : IHC-P
Reactivity : Mouse
Zhang ML et al. Biochim Biophys Acta 2017; iNOS-derived peroxynitrite mediates high glucose-induced inflammatory gene expression in vascular smooth muscle cells through promoting KLF5 expression and nitration.
Application : WB
Reactivity : Human, Mouse
Jiang W et al. Am J Transl Res 2016; 8 (11):4778-4790 Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages.
Application : WB, ICC/IF, IP
Reactivity : Mouse
Ma D et al. Biochem Biophys Res Commun 2016; (Epub) 1, 25(OH)2D3-induced interaction of vitamin D receptor with p50 subunit of NF-κB suppresses the interaction between KLF5 and p50, contributing to inhibition of LPS-induced macrophage proliferation.
Application : ICC/IF
Reactivity : Mouse
Zhao T et al. PLoS One 2015; 10 (9):e0138168 Roles of Klf5 Acetylation in the Self-Renewal and the Differentiation of Mouse Embryonic Stem Cells.
Application : WB
Reactivity : Mouse
Lappas M et al. Reproduction 2015; 149 (5):413-24 KLF5 regulates infection- and inflammation-induced pro-labour mediators in human myometrium.
Hu Z et al. Nat Genet 2015; 47 (2):158-63 Genome-wide profiling of HPV integration in cervical cancer identifies clustered genomic hot spots and a potential microhomology-mediated integration mechanism.
Application : IHC-P
Reactivity : Human
Zhang J et al. Biosci Rep 2014; Vascular calcification is coupled with phenotypic conversion of vascular smooth muscle cells through KLF5-mediated transactivation of the Runx2 promoter.
Application : WB
Reactivity : Rat
Chen HL et al. Mediators Inflamm. 2014; 2014:281984 Kruppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor-κB Phosphorylation In Vitro and In Vivo
Application : WB, IHC-P
Reactivity : Human, Mouse
Marrero-Rodriguez D et al. Tumour Biol. 2014; 35 (11):11399-407 Kr-ppel-like factor 5 as potential molecular marker in cervical cancer and the KLF family profile expression
Application : IHC-P
Reactivity : Human
Lv XR et al. Biochem J 2013; 456 (1):35-46 Synthetic retinoid Am80 up-regulates apelin expression by promoting interaction of RARα with KLF5 and Sp1 in vascular smooth muscle cells.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 419
$ 169