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KLF5 antibody

Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

Cat No. GTX103289

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

A549 , HCT116 , KLF5-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950625

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Kruppel like factor 5 , BTEB2 , CKLF , IKLF

Cellular Localization

Nucleus

Background

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103289

GTX103289 ICC/IF Image

KLF5 antibody detects KLF5 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KLF5 protein stained by KLF5 antibody (GTX103289) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
KLF5 antibody (GTX103289) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

KLF5 antibody Cat No. GTX103289

Rating ( Average 4.8 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 8 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 3 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014.PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biosci Rep in 2014.PMID: 25205373
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biosci Rep in 2014.PMID: 25205373
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014.PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014.PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014.PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014.PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biosci Rep in 2014.PMID: 25205373
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014.PMID: 25197166
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse heart tissue
Blocking : 3%BSA in PBST, 25°C, 1Hr
Fixation : 4%PFA in PBS
Primary Antibody : 1:1500, 4°C, 15Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf