GeneTex
  • Country / Location Selection

United States (US)

KLF5 antibody

Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289

Cat No. GTX103289

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

A549 , HCT116 , KLF5-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Kruppel like factor 5 , BTEB2 , CKLF , IKLF

Cellular Localization

Nucleus

Background

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103289

GTX103289 ICC/IF Image

KLF5 antibody detects KLF5 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KLF5 protein stained by KLF5 antibody (GTX103289) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
KLF5 antibody (GTX103289) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

KLF5 antibody Cat No. GTX103289

Rating ( Average 3 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse heart tissue
Blocking : 3%BSA in PBST, 25°C, 1Hr
Fixation : 4%PFA in PBS
Primary Antibody : 1:1500, 4°C, 15Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169