Non-transfected (–) and transfected (+) MDA-MB-231 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Kiss1 antibody [HL3043] (GTX640486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Kiss1 antibody [HL3043] detects secreted Kiss1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
Kiss1 stained by Kiss1 antibody [HL3043] (GTX640486) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min