Application Note
Immunoprecipitation (natured and denatured): use at a concentration of 2μg / mg. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2μg / ml for 30 min at RT. Optimal dilutions/concentrations should be determined by the end user. By Western blot, this antibody detects a band of 220-240 kDa, which corresponds to the predicted molecular weight of L1CAM. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Calculated MW
Positive Control
IMR-5 cells
Product Note
GTX23200 is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin. It does not bind to pediatric or adult brain.
Form
Liquid
Buffer
10mM PBS, 0.2% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Homogenous suspension of 16 week human fetal brain.
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_384977
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
L1 cell adhesion molecule , CAML1 , CD171 , HSAS , HSAS1 , MASA , MIC5 , N-CAM-L1 , N-CAML1 , NCAM-L1 , S10 , SPG1
Cellular Localization
Cell membrane , Cell projection, dendrite
Background
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]
Database
Research Area