Untreated (–) and treated (+) HeLa or HUVEC whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamp2A antibody (GTX132655) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP2A antibody (GTX132655) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) 293T whole cell and membrane extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lamp2A antibody (GTX132655) diluted at 1:1000. (ME: membrane extract)