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LDL Receptor antibody, Internal

Anti-LDL Receptor antibody, Internal used in Flow cytometry (FACS). GTX80914
Anti-LDL Receptor antibody, Internal used in Western Blot (WB). GTX80914

Cat. No. GTX80914

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all LDL Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Hamster, Pig, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 391-419 amino acids from the Central region of human LDLR.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11176679

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

low density lipoprotein receptor , FH , FHC , LDLCQ2

Cellular Localization

Cell membrane,Golgi apparatus,Lysosome

Background

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-LDL Receptor antibody, Internal used in Flow cytometry (FACS). GTX80914

GTX80914 FACS Image

FACS analysis of MCF-7 cells using GTX80914 LDL Receptor antibody, Internal.
Top histogram : negative control
Bottom histogram : MCF-7 cells

Anti-LDL Receptor antibody, Internal used in Western Blot (WB). GTX80914

GTX80914 WB Image

WB analysis of mouse lung tissue lysate (35ug/lane) using GTX80914 LDL Receptor antibody, Internal.

REFERENCE

Application Reference

Romagnuolo R et al. J Biol Chem 2015; 290 (18) : 11649-62 Lipoprotein(a) catabolism is regulated by proprotein convertase subtilisin/kexin type 9 through the low density lipoprotein receptor.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399