Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIM1 antibody [HL2774] (GTX639639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LIM1 antibody detects LIM1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: LIM1 stained by LIM1 antibody (GTX639639) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

LIM1 antibody detects LIM1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
LIM1 stained by LIM1 antibody (GTX639639) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min