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LIMP II antibody [GT1270]

Recombinant antibody
Anti-LIMP II antibody [GT1270] used in Western Blot (WB). GTX03182
Anti-LIMP II antibody [GT1270] used in Western Blot (WB). GTX03182
Anti-LIMP II antibody [GT1270] used in Western Blot (WB). GTX03182
Anti-LIMP II antibody [GT1270] used in IHC (Paraffin sections) (IHC-P). GTX03182
Anti-LIMP II antibody [GT1270] used in IHC (Paraffin sections) (IHC-P). GTX03182
Anti-LIMP II antibody [GT1270] used in IHC (Paraffin sections) (IHC-P). GTX03182

Cat. No. GTX03182

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1270

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all LIMP II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Observed MW

80 kDa. The observed M.W. is based on the following publication. PMID: 26219725, 31387993

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human LIMPII.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

scavenger receptor class B member 2 , AMRF , CD36L2 , EPM4 , HLGP85 , LGP85 , LIMP-2 , LIMPII , SR-BII

Cellular Localization

Lysosome membrane

Background

The protein encoded by this gene is a type III glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes. Earlier studies in mice and rat suggested that this protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. The protein deficiency in mice was reported to impair cell membrane transport processes and cause pelvic junction obstruction, deafness, and peripheral neuropathy. Further studies in human showed that this protein is a ubiquitously expressed protein and that it is involved in the pathogenesis of HFMD (hand, foot, and mouth disease) caused by enterovirus-71 and possibly by coxsackievirus A16. Mutations in this gene caused an autosomal recessive progressive myoclonic epilepsy-4 (EPM4), also known as action myoclonus-renal failure syndrome (AMRF). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-LIMP II antibody [GT1270] used in Western Blot (WB). GTX03182

GTX03182 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIMP II antibody [GT1270] (GTX03182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 26219725 and, PMID:31387993

Anti-LIMP II antibody [GT1270] used in Western Blot (WB). GTX03182

GTX03182 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIMP II antibody [GT1270] (GTX03182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 26219725 and, PMID:31387993

Anti-LIMP II antibody [GT1270] used in Western Blot (WB). GTX03182

GTX03182 WB Image

WB analysis of various samples using GTX03182 LIMP II antibody [GT1270].
Dilution : 1:1000
Loading : 25μg per lane

Anti-LIMP II antibody [GT1270] used in IHC (Paraffin sections) (IHC-P). GTX03182

GTX03182 IHC-P Image

IHC-P analysis of human brain tissue section using GTX03182 LIMP II antibody [GT1270].
Dilution : 1:100

Anti-LIMP II antibody [GT1270] used in IHC (Paraffin sections) (IHC-P). GTX03182

GTX03182 IHC-P Image

IHC-P analysis of mouse liver tissue section using GTX03182 LIMP II antibody [GT1270].
Dilution : 1:100

Anti-LIMP II antibody [GT1270] used in IHC (Paraffin sections) (IHC-P). GTX03182

GTX03182 IHC-P Image

IHC-P analysis of rat lung tissue section using GTX03182 LIMP II antibody [GT1270].
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439