APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
Observed MW
80 kDa. The observed M.W. is based on the following publication. PMID: 26219725, 31387993
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.34 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human LIMPII.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
scavenger receptor class B member 2 , AMRF , CD36L2 , EPM4 , HLGP85 , LGP85 , LIMP-2 , LIMPII , SR-BII
Cellular Localization
Lysosome membrane
Background
The protein encoded by this gene is a type III glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes. Earlier studies in mice and rat suggested that this protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. The protein deficiency in mice was reported to impair cell membrane transport processes and cause pelvic junction obstruction, deafness, and peripheral neuropathy. Further studies in human showed that this protein is a ubiquitously expressed protein and that it is involved in the pathogenesis of HFMD (hand, foot, and mouth disease) caused by enterovirus-71 and possibly by coxsackievirus A16. Mutations in this gene caused an autosomal recessive progressive myoclonic epilepsy-4 (EPM4), also known as action myoclonus-renal failure syndrome (AMRF). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX03182 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIMP II antibody [GT1270] (GTX03182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 26219725 and, PMID:31387993
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GTX03182 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIMP II antibody [GT1270] (GTX03182) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 26219725 and, PMID:31387993
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GTX03182 WB Image
WB analysis of various samples using GTX03182 LIMP II antibody [GT1270]. Dilution : 1:1000 Loading : 25μg per lane
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GTX03182 IHC-P Image
IHC-P analysis of human brain tissue section using GTX03182 LIMP II antibody [GT1270]. Dilution : 1:100
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GTX03182 IHC-P Image
IHC-P analysis of mouse liver tissue section using GTX03182 LIMP II antibody [GT1270]. Dilution : 1:100
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GTX03182 IHC-P Image
IHC-P analysis of rat lung tissue section using GTX03182 LIMP II antibody [GT1270]. Dilution : 1:100
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REFERENCE
There are currently no references for LIMP II antibody [GT1270] (GTX03182). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for LIMP II antibody [GT1270] (GTX03182). Be the first to share your experience with this product.