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LIN28B antibody

Anti-LIN28B antibody used in Western Blot (WB). GTX131546
Anti-LIN28B antibody used in IHC (Paraffin sections) (IHC-P). GTX131546
Anti-LIN28B antibody used in IHC (Paraffin sections) (IHC-P). GTX131546

Cat No. GTX131546

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Huh7

Predict Reactivity

Sheep(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LIN28B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

lin-28 homolog B,CSDD2

Cellular Localization

Cytoplasm , Nucleus

Background

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Database

Research Area

DATA IMAGES
Anti-LIN28B antibody used in Western Blot (WB). GTX131546

GTX131546 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LIN28B antibody (GTX131546) diluted at 1:1000.

Anti-LIN28B antibody used in IHC (Paraffin sections) (IHC-P). GTX131546

GTX131546 IHC-P Image

LIN28B antibody detects LIN28B protein at cytoplasm and nucleus in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
LIN28B antibody (GTX131546) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LIN28B antibody used in IHC (Paraffin sections) (IHC-P). GTX131546

GTX131546 IHC-P Image

LIN28B antibody detects LIN28B protein at cytoplasm and nucleus in human ovary by immunohistochemical analysis.
Sample: Paraffin-embedded human ovary.
LIN28B antibody (GTX131546) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169