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LMP2 antibody, C-term

Anti-LMP2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81175
Anti-LMP2 antibody, C-term used in Western Blot (WB). GTX81175
Anti-LMP2 antibody, C-term used in Western Blot (WB). GTX81175
Anti-LMP2 antibody, C-term used in Flow cytometry (FACS). GTX81175

Cat. No. GTX81175

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all LMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 193-219 amino acids from the C-terminal region of human PSMB9.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proteasome subunit beta 9 , LMP2 , PRAAS3 , PSMB6i , RING12 , beta1i

Cellular Localization

Cytoplasm,Nucleus

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-LMP2 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81175

GTX81175 IHC-P Image

IHC-P analysis of human prostate carcinoma using GTX81175 LMP2 antibody, C-term.

Anti-LMP2 antibody, C-term used in Western Blot (WB). GTX81175

GTX81175 WB Image

WB analysis of various samples using GTX81175 LMP2 antibody, C-term.
Lane 1: A431 whole cell lysate
Lane 2: Raji whole cell lysate
Loading : 20 μg per lane
Dilution : 1:1000

Anti-LMP2 antibody, C-term used in Western Blot (WB). GTX81175

GTX81175 WB Image

WB analysis of HL-60 cell lysate (35ug/lane) using GTX81175 LMP2 antibody, C-term.

Anti-LMP2 antibody, C-term used in Flow cytometry (FACS). GTX81175

GTX81175 FACS Image

FACS analysis of HL-60 cells using GTX81175 LMP2 antibody, C-term.
Top histogram : negative control
Bottom histogram : HL-60 cells

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399