Name: LOXL2 antibody
Brand: GeneTex
SKU: GTX105085
Price: 419.00 USD

GTX105085 WB Image

Wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX105085 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

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GTX105085 WB Image

Human LOXL2 protein, His tag (0.05 μg, GTX136930-pro) was separated by 10% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX105085 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000.

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GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX105085 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Human LOXL2 protein, His tag (GTX136930-pro) (44.94-0.7 nM). Coated protein was probed with LOXL2 antibody (GTX105085) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

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GTX105085 IHC-P Image

LOXL2 antibody detects LOXL2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:3000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500..
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

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GTX105085 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX105085 ICC/IF Image

LOXL2 antibody detects LOXL2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

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