LRP2 antibody [ARC63830]

Cat. No. GTX04758

Host	Rabbit
Clonality	Monoclonal
Clone Name	ARC63830
Isotype	IgG
Application	WB, IHC-P, FACS
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all LRP2 products

Datasheet

View product citations for antibody GTX04758 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:1000
IHC-P	1:50-1:200
FACS	1:100-1:500

Not tested in other applications.

Calculated MW

522 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% glycerol

Preservative

0.05% proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human intracellular Megalin/LRP2 (NP_004516.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DBS,GP330,LDL receptor related protein 2,LRP2,Lrp2,Megalin

Cellular Localization

Membrane,Coated pit

Background

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009]

Database

Gene ID: 4036 LRP2
UniProt: P98164 LRP2

Research Area

DATA IMAGES

GTX04758 IHC-P Image

IHC-P analysis of human kidney tissue using GTX04758 LRP2 antibody [ARC63830].
Antigen retrieval : 10 mM citrate buffer (pH 6.0) with high pressure
Dilution : 1:200

GTX04758 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04758 LRP2 antibody [ARC63830].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:200

GTX04758 FACS Image

FACS analysis of Reh (left panel) and Caco-2 (right panel) cells using GTX04758 LRP2 antibody [ARC63830].
Orange : Primary antibody
Blue : Isotype control
Red : Unlabeled cells
Dilution : 2 μg/mL for 10⁶ cells

GTX04758 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04758 LRP2 antibody [ARC63830].
Antigen retrieval : 10 mM citrate buffer (pH 6.0) with high pressure
Dilution : 1:200

GTX04758 FACS Image

FACS analysis of Caco-2 cells using GTX04758 LRP2 antibody [ARC63830].
Left panel : Isotype control
Right panel : Primary antibody
Dilution : 2 μg/mL for 10⁶ cells

GTX04758 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04758 LRP2 antibody [ARC63830].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:200

GTX04758 WB Image

WB analysis of mouse kidney tissue lysate using GTX04758 LRP2 antibody [ARC63830].
Loading : 25 μg
Dilution : 1:1000

GTX04758 IHC-P Image

IHC-P analysis of rat liver tissue using GTX04758 LRP2 antibody [ARC63830].
Antigen retrieval : 10 mM citrate buffer (pH 6.0) with high pressure
Dilution : 1:200

GTX04758 IHC-P Image

IHC-P analysis of human liver tissue using GTX04758 LRP2 antibody [ARC63830].
Antigen retrieval : 10 mM citrate buffer (pH 6.0) with high pressure
Dilution : 1:200

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

Qty

LRP2 antibody [ARC63830]

Cat. No. GTX04758

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX04758 IHC-P Image

GTX04758 IHC-P Image

GTX04758 FACS Image

GTX04758 IHC-P Image

GTX04758 FACS Image

GTX04758 IHC-P Image

GTX04758 WB Image

GTX04758 IHC-P Image

GTX04758 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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