Non-transfected (–) and transfected (+) boiled and unboiled 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LRRC15 antibody [N1N3] (GTX120895) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled U87-MG whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LRRC15 antibody [N1N3] (GTX120895) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)