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LSM14A antibody

Anti-LSM14A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120187
Anti-LSM14A antibody used in Western Blot (WB). GTX120187
Anti-LSM14A antibody used in IHC (Paraffin sections) (IHC-P). GTX120187
Anti-LSM14A antibody used in Western Blot (WB). GTX120187
Anti-LSM14A antibody used in IHC (Paraffin sections) (IHC-P). GTX120187

Cat No. GTX120187

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , NT2D1

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LSM14A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LSM14A mRNA processing body assembly factor , C19orf13 , FAM61A , RAP55 , RAP55A

Background

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM]

Database

DATA IMAGES

Anti-LSM14A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120187

GTX120187 ICC/IF Image

LSM14A antibody detects LSM14A protein at P-body by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LSM14A protein stained by LSM14A antibody (GTX120187) diluted at 1:500.
Red: alpha Tubulin , a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-LSM14A antibody used in Western Blot (WB). GTX120187

GTX120187 WB Image

LSM14A antibody detects LSM14A protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LSM14A antibody (GTX120187) diluted by 1:1000.

Anti-LSM14A antibody used in IHC (Paraffin sections) (IHC-P). GTX120187

GTX120187 IHC-P Image

LSM14A antibody detects LSM14A protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
LSM14A antibody (GTX120187) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LSM14A antibody used in Western Blot (WB). GTX120187

GTX120187 WB Image

LSM14A antibody detects LSM14A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LSM14A antibody (GTX120187) diluted by 1:1000.

Anti-LSM14A antibody used in IHC (Paraffin sections) (IHC-P). GTX120187

GTX120187 IHC-P Image

LSM14A antibody detects LSM14A protein at cytosol on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
LSM14A antibody (GTX120187) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169