GeneTex
  • Country / Location Selection

United States (US)

Lac I antibody

Anti-Lac I antibody used in Western Blot (WB). GTX48707

Cat No. GTX48707

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity E. coli, Shigella sonnei
Package
50 μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ELISA 1:90000 – 1:100000
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.

Research Area

DATA IMAGES
Anti-Lac I antibody used in Western Blot (WB). GTX48707

GTX48707 WB Image

Western blot using GeneTex's affinity purified anti-Lac I antibody shows detection of a 38 kDa band corresponding to recombinant Lac I (arrowhead). The blot was blocked with 5% BLOTTO in TBST overnight at 4°C. Primary antibody was used at a 1:1,000 dilution followed by reaction with a 1:10,000 dilution of HRP goat anti-rabbit IgG. ECL was used for detection.

REFERENCE
There are currently no references for Lac I antibody (GTX48707). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Lac I antibody (GTX48707). Be the first to share your experience with this product.
Package List Price ($)
$ 319