Human milk (5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lactoferrin antibody (GTX113488) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using Lactoferrin(GTX113488) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min