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Lamin A + C antibody

KO/KD Comparison Orthogonal
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Anti-Lamin A + C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101127
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127
Anti-Lamin A + C antibody used in Immunoprecipitation (IP). GTX101127
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127

Cat. No. GTX101127

Reference 42
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Lamin A + C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

NIH-3T3

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729290

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

Cellular Localization

Nucleus

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127

GTX101127 WB Image

KO/KD

Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lamin A + C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101127

GTX101127 ICC/IF Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Lamin A + C stained by Lamin A + C antibody (GTX101127) diluted at 1:1000.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127

GTX101127 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Lamin A + C antibody (GTX101127) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127

GTX101127 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127

GTX101127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127

GTX101127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in Immunoprecipitation (IP). GTX101127

GTX101127 IP Image

Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.
IP samples: HeLa whole cell extract
A. 50 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Lamin A + C protein by 4 μg Lamin A + C antibody (GTX101127)
7.5 % SDS-PAGE
The immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (GTX101127) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127

GTX101127 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Lamin A + C antibody (GTX101127) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127

GTX101127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127

GTX101127 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX101127 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127

GTX101127 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127

GTX101127 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Lamin A + C antibody used in IHC (Paraffin sections) (IHC-P). GTX101127

GTX101127 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Lamin A + C stained by Lamin A + C antibody (GTX101127) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kamil So?tysik et al. J Cell Biol 2021; 220 (1) Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner.
Application : WB
Reactivity : Human
Wei-Lun Liu et al. Int J Mol Sci 2021; 22 (7) High Mobility Group Box 1 Promotes Lung Cancer Cell Migration and Motility via Regulation of Dynamin-Related Protein 1.
Application : WB
Reactivity : Human
Ming-Tsung Wu et al. Int J Mol Sci 2021; 22 (17) MTHFR Knockdown Assists Cell Defense against Folate Depletion Induced Chromosome Segregation and Uracil Misincorporation in DNA.
Application : WB
Reactivity : Human
Conte M et al. Int J Mol Sci 2021; 23 (1) Relevance of AIF/CypA Lethal Pathway in SH-SY5Y Cells Treated with Staurosporine.
Application : WB
Reactivity : Human
Wu PS et al. Biomedicines 2021; 9 (12) 8-Hydroxydaidzein Downregulates JAK/STAT, MMP, Oxidative Phosphorylation, and PI3K/AKT Pathways in K562 Cells.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Lamin A + C antibody Cat. No. GTX101127

Rating
( Average 4.6 based on 5 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Lamin A + C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101127
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2016. PMID: 27483354
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014. PMID: 25122478
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Date : Anonymous submitted on 28-Oct-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : T24 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in PBST, 37°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Date : Anonymous submitted on 13-Mar-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 15 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 25°C, 2Hr
Lane Description : Lane 1: A549 cytosol extract, Lane 2: A549 nuclear extract
Anti-Lamin A + C antibody used in Western Blot (WB). GTX101127
Date : Anonymous submitted on 28-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : K562 cells
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane 1: K562 cytosol extract. Lane 2: K562 nuclear extract.
Submit a Review
Application Reference
Kamil So?tysik et al. J Cell Biol 2021; 220 (1) Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner.
Application : WB
Reactivity : Human
Wei-Lun Liu et al. Int J Mol Sci 2021; 22 (7) High Mobility Group Box 1 Promotes Lung Cancer Cell Migration and Motility via Regulation of Dynamin-Related Protein 1.
Application : WB
Reactivity : Human
Ming-Tsung Wu et al. Int J Mol Sci 2021; 22 (17) MTHFR Knockdown Assists Cell Defense against Folate Depletion Induced Chromosome Segregation and Uracil Misincorporation in DNA.
Application : WB
Reactivity : Human
Conte M et al. Int J Mol Sci 2021; 23 (1) Relevance of AIF/CypA Lethal Pathway in SH-SY5Y Cells Treated with Staurosporine.
Application : WB
Reactivity : Human
Wu PS et al. Biomedicines 2021; 9 (12) 8-Hydroxydaidzein Downregulates JAK/STAT, MMP, Oxidative Phosphorylation, and PI3K/AKT Pathways in K562 Cells.
Application : WB
Reactivity : Human
Jung F et al. Pharmacol Res Perspect 2021; 9 (6):e00876 FJU-C28 inhibits the endotoxin-induced pro-inflammatory cytokines expression via suppressing JNK, p38 MAPK and NF-κB signaling pathways.
Application : WB
Reactivity : Mouse
Hua HS et al. J Biomed Sci 2021; 28 (1):38 Histone deacetylase 7 mediates endothelin-1-induced connective tissue growth factor expression in human lung fibroblasts through p300 and activator protein-1 activation.
Application : WB
Reactivity : Human
Hsu SC et al. Theranostics 2021; 11 (15):7527-7545 Arginine starvation elicits chromatin leakage and cGAS-STING activation via epigenetic silencing of metabolic and DNA-repair genes.
Application : WB
Reactivity : Human
Teng YN et al. Cells 2021; 10 (6) Etoposide Triggers Cellular Senescence by Inducing Multiple Centrosomes and Primary Cilia in Adrenocortical Tumor Cells.
Application : WB
Reactivity : Mouse
Wu PS et al. Biomedicines 2020; 8 (11) 8-Hydroxydaidzein, an Isoflavone from Fermented Soybean, Induces Autophagy, Apoptosis, Differentiation, and Degradation of Oncoprotein BCR-ABL in K562 Cells.
Application : WB
Reactivity : Human
Garrido-Pascual P et al. Stem Cell Res Ther 2020; 11 (1):335 H2O2-preconditioned human adipose-derived stem cells (HC016) increase their resistance to oxidative stress by overexpressing Nrf2 and bioenergetic adaptation.
Application : WB
Reactivity : Human
Sakuratani T et al. Virchows Arch 2020; (Epub) Downregulation of ARID1A in gastric cancer cells: a putative protective molecular mechanism against the Harakiri-mediated apoptosis pathway.
Application : WB
Reactivity : Human
Chen YC et al. Part Fibre Toxicol 2020; 17 (1):41 Particulate matters increase epithelial-mesenchymal transition and lung fibrosis through the ETS-1/NF-κB-dependent pathway in lung epithelial cells.
Application : WB
Reactivity : Human
Zhang Y et al. Front Physiol 2020; 11:510 MiR-181d-5p Targets KLF6 to Improve Ischemia/Reperfusion-Induced AKI Through Effects on Renal Function, Apoptosis, and Inflammation.
Application : WB
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Juen-HaurHwang et al. Journal of Functional Foods 2020; 66:103832 Hericium erinaceus enhances neurotrophic factors and prevents cochlear cell apoptosis in senescence accelerated mice.
Application : WB
Kuo YP et al. RNA Biol 2019; 16 (9):1263-1274 A novel antisense RNA ASPACT confers multi-level suppression of PACT and associated signalling.
Bueno C et al. Sci Rep 2019; 9 (1):18038 Non-proliferative neurogenesis in human periodontal ligament stem cells.
Kuo YY et al. Cell Commun Signal 2019; 17 (1):100 Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81 and Ser213.
Application : WB
Reactivity : Human
Takano M et al. Life Sci 2019; 229:173-179 Role of peptide transporter 2 and MAPK signaling pathways in the innate immune response induced by bacterial peptides in alveolar epithelial cells.
Application : WB
Reactivity : Human
Kuo CC et al. Theranostics 2019; 9 (9):2526-2540 Metastatic Colorectal Cancer Rewrites Metabolic Program Through a Glut3-YAP-dependent Signaling Circuit.
Application : WB
Reactivity : Human
Yu HI et al. J Pharm Biomed Anal 2018; 160:344-350 Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Lee WY et al. Int J Cancer 2017; 141 (9):1921-1931 * sensitizes kras-mutant non-small-cell lung cancer to * by targeting taz.
Application : WB
Reactivity : Human
Hung YL et al. Sci Rep 2017; 7:46299 Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response.
Reactivity : Mouse
Singh N et al. Dis Model Mech 2016; 9 (8):885-97 Alterations in nuclear structure promote lupus autoimmunity in a mouse model.
Application : WB, ICC/IF
Reactivity : Human
Ling HH et al. Exp Cell Res 2016; Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.
Application : WB
Reactivity : Human
Yang SY et al. Sci Rep 2016; 6:32787 A low-salt diet increases the expression of renal sirtuin 1 through activation of the ghrelin receptor in rats.
Application : WB
Reactivity : Rat
Singh M et al. Dis Model Mech 2016; (Epub) Alterations in nuclear structure promote lupus autoimmunity in a mouse model.
Application : ICC/IF, IP
Reactivity : Human
Chen HH et al. Sci Rep 2016; 6:27945 Urinary miR-16 transactivated by C/EBPβ reduces kidney function after ischemia/reperfusion-induced injury.
Application : WB
Reactivity : Mouse
Kuo HF et al. PLoS One 2016; 11 (5):e0156059 Pigment Epithelium-Derived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury.
Application : WB
Reactivity : Human
Kadono N et al. Mol Med 2015; 21:77-86 Extracellularly Extruded Syntaxin-4 Is a Potent Cornification Regulator of Epidermal Keratinocytes.
Application : WB
Reactivity : Human
Wu PS et al. PLoS One 2015; 10 (6):e0130599 Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.
Lin H et al. Mol Pharmacol 2014; 85 (5):682-91 Activating transcription factor 3 protects against pressure-overload heart failure via the autophagy molecule Beclin-1 pathway.
Application : WB
Reactivity : Mouse
Anacker DC et al. J Virol 2014; 88 (15):8528-44 Productive replication of human papillomavirus 31 requires DNA repair factor Nbs1.
Application : WB
Reactivity : Human
Chen HL et al. Mediators Inflamm. 2014; 2014:281984 Kruppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor-κB Phosphorylation In Vitro and In Vivo
Application : WB
Reactivity : Human
Lien GS et al. PLoS One 2014; 9 (8):e104891 Epidermal Growth Factor Stimulates Nuclear Factor-κB Activation and Heme Oxygenase-1 Expression via c-Src, NADPH Oxidase, PI3K, and Akt in Human Colon Cancer Cells.
Application : WB
Reactivity : Human
Galloway JN et al. Hum Mol Genet 2014; CGG repeats in RNA modulate expression of TDP-43 in mouse and fly models of fragile X tremor ataxia syndrome.
Application : IHC-P
Reactivity : Mouse
Lee CF et al. J Biol Chem 2014; 289 (3):1257-70 The A2A adenosine receptor is a dual coding gene: a novel mechanism of gene usage and signal transduction.
Application : WB
Reactivity : Rat
Liu JS et al. PLoS One 2013; 8 (12):e82877 FJU-C4, a new 2-pyridone compound, attenuates lipopolysaccharide-induced systemic inflammation via p38MAPK and NF-κB in mice.
Application : WB
Reactivity : Mouse
Lin H et al. Mol Pharmacol 2013; 84 (2):275-85 Adiponectin ameliorates iron-overload cardiomyopathy through the PPARα-PGC-1-dependent signaling pathway.
Application : WB
Reactivity : Rat
Chen YS et al. Biochem Biophys Res Commun 2013; 440 (4):671-6 Identification of the proteins required for fatty acid desaturation in zebrafish (Danio rerio).
Application : WB
Reactivity : Human
Hsin IL et al. Toxicol Appl Pharmacol 2012; 263 (3):330-7 Lipocalin 2, a new GADD153 target gene, as an apoptosis inducer of endoplasmic reticulum stress in lung cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169