APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3
Predict Reactivity
Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.16 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Lamin A + C. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10729290
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1
Cellular Localization
Nucleus
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]
Database
Research Area
DATA IMAGES
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GTX101127 WB Image
Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101127 ICC/IF Image
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Lamin A + C stained by Lamin A + C antibody (GTX101127) diluted at 1:1000.Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX101127 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX101127 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Lamin A + C antibody (GTX101127) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX101127 IP Image
Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.
IP samples: HeLa whole cell extract
A. 50 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Lamin A + C protein by 4 μg Lamin A + C antibody (GTX101127)
7.5 % SDS-PAGE
The immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (GTX101127) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX101127 IHC-P Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101127 WB Image
Sample (30 μg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX101127 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101127 IHC-P Image
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101127 IHC-P Image
Lamin A + C antibody detects Lamin A + C protein at nuclear envelope in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Lamin A + C antibody (GTX101127) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101127 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101127 IHC-P Image
Lamin A + C antibody detects Lamin A + C protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Lamin A + C stained by Lamin A + C antibody (GTX101127) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101127 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101127 ICC/IF Image
The data was published in the journal Dis Model Mech in 2016. PMID: 27483354
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GTX101127 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25122478
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REFERENCE
REVIEW
Lamin A + C antibody
Cat. No. GTX101127
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Rating
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( Average 4.3 based on 3 users reviews)
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Application
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Western Blot(WB)
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( Average 4.3 based on 3 users reviews)
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Date :
Anonymous submitted on 28-Oct-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
T24 cells
Amount used :
30 μg
Blocking :
5% non-fat milk in PBST, 37°C, 1.5Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 13-Mar-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
15 μg
Blocking :
5% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:3000, 25°C, 2Hr
Lane Description :
Lane 1: A549 cytosol extract, Lane 2: A549 nuclear extract
Date :
Anonymous submitted on 28-May-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
K562 cells
Amount used :
10μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
Lane Description :
Lane 1: K562 cytosol extract. Lane 2: K562 nuclear extract.
