GeneTex
United States (US)

Lamin A + C antibody

KO/KD
GTX101127_41815_20170323_WB_KO_watermark_19011506_357.jpg
GTX101127_40401_IHC_M_19011506_106.jpg
GTX101127_40401_IHC_R_19011506_211.jpg
GTX101127_41815_WB_19011506_477.jpg
GTX101127_40401_IP_19011506_485.jpg
GTX101127_40401_IFA_19011506_824.jpg
GTX101127_41815_20151227_IHC-P_19011506_273.jpg
GTX101127_40401_IHC_19011506_705.jpg
GTX101127_40401_WB_M_19011506_519.jpg

Cat No. GTX101127

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa , A431 , NIH-3T3 , *K562

Predict Reactivity

Bovine, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lamin A/C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lamin A/C,Cdcd1,Cddc,Cmd1A,Cmt2B1,Emd2,Fpl,Fpld,Fpld2,Hgps,Idc,Ldp1,Lfp,Lgmd1B,Lmn1,Lmnc,Lmnl1,Mada,Pro1,Lmna

Cellular Localization

Nucleus

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES
GTX101127_41815_20170323_WB_KO_watermark_19011506_357.jpg

GTX101127 WB Image

KO/KD

Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101127_40401_IHC_M_19011506_106.jpg

GTX101127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101127_40401_IHC_R_19011506_211.jpg

GTX101127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101127_41815_WB_19011506_477.jpg

GTX101127 WB Image

Lamin A + C antibody detects Lamin A + C protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin A + C antibody (GTX101127) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101127_40401_IP_19011506_485.jpg

GTX101127 IP Image

Lamin A + C antibody immunoprecipitates Lamin A + C protein in IP experiments.
IP samples: HeLa whole cell extract
A. 50 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Lamin A + C protein by 4 μg Lamin A + C antibody (GTX101127)
7.5 % SDS-PAGE
The immunoprecipitated Lamin A + C protein was detected by Lamin A + C antibody (GTX101127) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX101127_40401_IFA_19011506_824.jpg

GTX101127 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Lamin A + C(GTX101127) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

GTX101127_41815_20151227_IHC-P_19011506_273.jpg

GTX101127 IHC-P Image

Lamin A + C antibody detects Lamin A + C protein at nuclear envelope in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Lamin A + C antibody (GTX101127) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101127_40401_IHC_19011506_705.jpg

GTX101127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using Lamin A + C(GTX101127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101127_40401_WB_M_19011506_519.jpg

GTX101127 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX101127 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Lamin A + C antibody Cat No. GTX101127

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 13-Mar-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 15 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 25°C, 2Hr
Lane Description : Lane 1: A549 cytosol extract, Lane 2: A549 nuclear extract
Date : Anonymous submitted on 28-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : K562 cells
Amount used : 10μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : Lane 1: K562 cytosol extract. Lane 2: K562 nuclear extract.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169