Name: Laminin alpha 3B antibody [F7]
Brand: GeneTex
SKU: GTX17684
Price: 419.00 USD

Laminin alpha 3B antibody [F7]

Cat. No. GTX17684

References 3

Cat. No. GTX17684

GTX17684 Image

Protein structure of laminin-332s and binding sites of antibodies

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GTX17684 ELISA Image

ELISA anaiysis of five recombinant human laminins using GTX17684 Laminin alpha 3B antibody [F7] and GTX17685 Laminin alpha 3A antibody [BG5]. Clone F7 specifically detects Lm3B32 isoform.

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GTX17684 IHC-P Image

IHC-P analysis of various tissue samples using GTX17684 Laminin alpha 3B antibody [F7] (Black arrows : epithelial basement membranes ; Arrow heads : vascular basement membrane).
Figure A : Normal skin tissue (paraffin slice)
Figure B : Normal esophagus tissue (paraffin slice)
Figure C : Normal mammary gland (paraffin slice)
Figure D : Normal lung tissue (paraffin slice)

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GTX17684 WB Image

WB analysis of recombinant human intact laminin-3B32 (Lm3B32 , containing alpha 3B, Beta 3 and gamma 2 chains) using GTX17684 Laminin alpha 3B antibody [F7].
Dilution : 1:10000
Reduced condition : 7% gel
Non-reduced condition : 5% gel

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GTX17684 IHC-P Image

IHC-P analysis of skin cancer tissue using GTX17684 Laminin alpha 3B antibody [F7].

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Host

Mouse
Clonality

Monoclonal
Clone Name

F7
Isotype

IgG1
Applications

WB IHC-P IHC-Fr IP ELISA Purification
Reactivity

Human

Summary

Laminins, which consist of three subunits called alpha, beta and gamma chains, are major cell-adhesive components of extracellular matrix, especially basement membranes (BMs). The laminin family is constituted of over 15 isoforms, and each member is expressed in a tissue-specific manner and plays a differential role in each tissue. In the case of laminin alpha 3 chain, there are two splicing variants, the truncated form alpha 3A and the full-length alpha 3B. Laminin-3B32 (Lm3B32) (formerly, laminin-5B) is composed of alpha 3B, beta 3 and gamma 2 chains and less widely expressed than laminin-3A32. Although laminin-3B32 shows higher cell adhesion activity than laminin-3A32 in vitro, differences of biological functions between two laminins remain to be clarified. Recent studies identified laminin-3B11 (Lm3B11) as a new alpha 3B-type laminin. Laminin-3B11 is localized in vascular basement membranes in normal tissues, but this expression is down-regulated in cancer tissues.Laminin-3B11 stimulates microvascular endothelial cells to extend lamelliopodial protrusions. This antibody (clone F7) detects laiminin-3B32 in the basement membranes of normal epithelial tissues and of relatively benign or differentiated carcinomas and laminin-3B11 in normal vascular basement membranes. This antibody is a powerful tool to detect laminin alpha 3B isoform and investigate its fundamental functions in epithelial and vascular basement membranes.