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Laminin beta 1 antibody

Anti-Laminin beta 1 antibody used in Western Blot (WB). GTX100787
Anti-Laminin beta 1 antibody used in Western Blot (WB). GTX100787
Anti-Laminin beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100787
Anti-Laminin beta 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100787

Cat No. GTX100787

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , NIH-3T3

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Laminin beta 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

laminin subunit beta 1 , CLM , LIS5

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane

Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Laminin beta 1 antibody used in Western Blot (WB). GTX100787

GTX100787 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
5% SDS PAGE
GTX100787 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Laminin beta 1 antibody used in Western Blot (WB). GTX100787

GTX100787 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
5% SDS PAGE
GTX100787 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Laminin beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100787

GTX100787 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Laminin beta 1(GTX100787) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Laminin beta 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100787

GTX100787 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Laminin beta-1(GTX100787) antibody at 1:100 dilution.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169