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Lcp1 antibody

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420
Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420
Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

Cat. No. GTX124420

Reference 14
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Lcp1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Zebrafish (100%)| Chicken (82%)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.54 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Lcp1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167454

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lymphocyte cytosolic protein 1 (L-plastin) , cb245 , fj24b12 , wu:fj24b12

Cellular Localization

Cytoplasm

Background

Actin-binding protein. Plays a role in the activation of T-cells.

Database

Research Area

DATA IMAGES

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Lcp1 antibody (GTX124420) dilution: 1:100.

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Lcp1 stained by Lcp1 antibody (GTX124420) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Lcp1 stained by Lcp1 antibody (GTX124420) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

Application Reference

Lindsey T Lelieveld et al. J Lipid Res 2022; 63 (5) : 100199 Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish.
Application : IHC-P
Reactivity : Zebrafish
Laura Gence et al. Front Pharmacol 2022; 13 : 832928 Hypericum lanceolatum Lam. Medicinal Plant: Potential Toxicity and Therapeutic Effects Based on a Zebrafish Model.
Zachary J Brandt et al. Invest Ophthalmol Vis Sci 2021; 62 (15) : 13 Ablation of mpeg+ Macrophages Exacerbates mfrp-Related Hyperopia.
Application : IHC-P
Reactivity : Zebrafish
Schmitner N et al. Cells 2021; 10 (11) Differential Responses of Neural Retina Progenitor Populations to Chronic Hyperglycemia.
Application : IHC-Wm
Reactivity : Zebrafish
Van Houcke J et al. Aging Cell 2021; 20 (9) : e13464 Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish Nothobranchius furzeri.
Application : IHC-Fr
Show More +
Submit a Reference

REVIEW

Lcp1 antibody Cat. No. GTX124420

Rating
( Average 5 based on 4 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-Lcp1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124420
Date : Anonymous submitted on 07-Jun-2022
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Sample : Zebrafish brain tissue section
Blocking : Goat serum, 37°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.0.1M citrate buffer (pH 6.0)
Primary Antibody : 1:100, 4°C, 48Hr
Anti-Lcp1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124420
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28871112
Anti-Lcp1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124420
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 30143654
Anti-Lcp1 antibody used in Immunohistochemistry (IHC). GTX124420
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 30143654
Submit a Review
Application Reference
Lindsey T Lelieveld et al. J Lipid Res 2022; 63 (5):100199 Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish.
Application : IHC-P
Reactivity : Zebrafish
Laura Gence et al. Front Pharmacol 2022; 13:832928 Hypericum lanceolatum Lam. Medicinal Plant: Potential Toxicity and Therapeutic Effects Based on a Zebrafish Model.
Zachary J Brandt et al. Invest Ophthalmol Vis Sci 2021; 62 (15):13 Ablation of mpeg+ Macrophages Exacerbates mfrp-Related Hyperopia.
Application : IHC-P
Reactivity : Zebrafish
Schmitner N et al. Cells 2021; 10 (11) Differential Responses of Neural Retina Progenitor Populations to Chronic Hyperglycemia.
Application : IHC-Wm
Reactivity : Zebrafish
Vanhunsel S et al. NPJ Aging Mech Dis 2021; 7 (1):22 The killifish visual system as an in vivo model to study brain aging and rejuvenation.
Application : IHC-Fr
Van Houcke J et al. Aging Cell 2021; 20 (9):e13464 Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish Nothobranchius furzeri.
Application : IHC-Fr
Beckers A et al. Neuroscience 2021; 470:52-69 Injury-induced Autophagy Delays Axonal Regeneration after Optic Nerve Damage in Adult Zebrafish.
Application : IHC
Reactivity : Zebrafish
Houcke J.V. et al. bioRxiv 2021; (Epub) Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish N. furzeri.
Application : IHC-Fr
Reactivity : Zebrafish
Ping Song et al. Front Cell Dev Biol 2020; 8:578528 Cone Photoreceptor Degeneration and Neuroinflammation in the Zebrafish Bardet-Biedl Syndrome 2 (bbs2) Mutant Does Not Lead to Retinal Regeneration.
Application : IHC-Fr
Reactivity : Zebrafish
Martins RR et al. Front Cell Dev Biol 2019; 7:12 Resident Immunity in Tissue Repair and Maintenance: The Zebrafish Model Coming of Age.
Lahne M et al. Exp Eye Res 2018; 178:148-159 Photo-regulation of rod precursor cell proliferation.
Reactivity : Zebrafish
Lopez-Munoz A et al. Sci Rep 2018; 8 (1):12802 An Adult Zebrafish Model Reveals that Mucormycosis Induces Apoptosis of Infected Macrophages.
Reactivity : Zebrafish
Yang Q et al. Sci Rep 2017; 7 (1):10315 Activation of liver stromal cells is associated with male-biased liver tumor initiation in xmrk and Myc transgenic zebrafish.
Reactivity : Zebrafish
Han P et al. Cell Res 2014; 24 (9):1091-107 Hydrogen peroxide primes heart regeneration with a derepression mechanism.
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169