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Lcp1 antibody

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420
Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420
Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

Cat. No. GTX124420

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Zebrafish (100%)| Chicken (82%)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.54 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Lcp1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167454

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lymphocyte cytosolic protein 1 (L-plastin) , cb245 , fj24b12 , wu:fj24b12

Cellular Localization

Cytoplasm

Background

Actin-binding protein. Plays a role in the activation of T-cells.

Database

Research Area

DATA IMAGES

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Lcp1 antibody (GTX124420) dilution: 1:100.

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Lcp1 stained by Lcp1 antibody (GTX124420) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Lcp1 stained by Lcp1 antibody (GTX124420) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

REVIEW

Lcp1 antibody Cat. No. GTX124420

Rating ( Average 5 based on 4 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 07-Jun-2022
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Sample : Zebrafish brain tissue section
Blocking : Goat serum, 37°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.0.1M citrate buffer (pH 6.0)
Primary Antibody : 1:100, 4°C, 48Hr
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28871112
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 30143654
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 30143654
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169