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Lcp1 antibody

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420
Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420
Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

Cat. No. GTX124420

Reference 20
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Lcp1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Zebrafish (100%)| Chicken (82%)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Lcp1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167454

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphocyte cytosolic protein 1 (L-plastin) , cb245 , fj24b12 , wu:fj24b12

Cellular Localization

Cytoplasm

Background

Actin-binding protein. Plays a role in the activation of T-cells.

Database

Research Area

DATA IMAGES

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Lcp1 stained by Lcp1 antibody (GTX124420) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Lcp1 stained by Lcp1 antibody (GTX124420) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Lcp1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124420

GTX124420 IHC-Wm Image

Lcp1 antibody detects Lcp1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Lcp1 antibody (GTX124420) dilution: 1:100.

REFERENCE

Application Reference

M El Ma? et al. Nat Aging 2023; Gut-specific telomerase expression counteracts systemic aging in telomerase-deficient zebrafish
Application : IHC-Fr
Reactivity : Zebrafish
Zhao F et al. Nat Commun 2022; 13 (1) : 6117 Brain milieu induces early microglial maturation through the BAX-Notch axis.
Wang J et al. Dis Model Mech 2022; 15 (12) Variable phenotypes and penetrance between and within different zebrafish ciliary transition zone mutants.
Application : IHC-Fr
Reactivity : Zebrafish
Li X et al. Cell Death Discov 2022; 8 (1) : 323 Estrogens revert neutrophil hyperplasia by inhibiting Hif1α-cMyb pathway in zebrafish myelodysplastic syndromes models.
Ellis PS et al. Immun Ageing 2022; 19 (1) : 31 A subset of gut leukocytes has telomerase-dependent "hyper-long" telomeres and require telomerase for function in zebrafish.
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Submit a Reference

REVIEW

Lcp1 antibody Cat. No. GTX124420

Rating
( Average 5 based on 4 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC)
( Average 5 based on 1 users reviews)
Anti-Lcp1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124420
Date : Anonymous submitted on 07-Jun-2022
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Sample : Zebrafish brain tissue section
Blocking : Goat serum, 37°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.0.1M citrate buffer (pH 6.0)
Primary Antibody : 1:100, 4°C, 48Hr
Anti-Lcp1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124420
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28871112
Anti-Lcp1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124420
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 30143654
Anti-Lcp1 antibody used in Immunohistochemistry (IHC). GTX124420
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 30143654
Submit a Review
Application Reference
M El Ma? et al. Nat Aging 2023; Gut-specific telomerase expression counteracts systemic aging in telomerase-deficient zebrafish
Application : IHC-Fr
Reactivity : Zebrafish
Fogerty J et al. J Neurosci 2022; 42 (26):5144-5158 Notch Inhibition Promotes Regeneration and Immunosuppression Supports Cone Survival in a Zebrafish Model of Inherited Retinal Dystrophy.
Application : IHC-Fr
Reactivity : Zebrafish
Zhao F et al. Nat Commun 2022; 13 (1):6117 Brain milieu induces early microglial maturation through the BAX-Notch axis.
Wang J et al. Dis Model Mech 2022; 15 (12) Variable phenotypes and penetrance between and within different zebrafish ciliary transition zone mutants.
Application : IHC-Fr
Reactivity : Zebrafish
Li X et al. Cell Death Discov 2022; 8 (1):323 Estrogens revert neutrophil hyperplasia by inhibiting Hif1α-cMyb pathway in zebrafish myelodysplastic syndromes models.
Ellis PS et al. Immun Ageing 2022; 19 (1):31 A subset of gut leukocytes has telomerase-dependent "hyper-long" telomeres and require telomerase for function in zebrafish.
Lindsey T Lelieveld et al. J Lipid Res 2022; 63 (5):100199 Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish.
Application : IHC-P
Reactivity : Zebrafish
Laura Gence et al. Front Pharmacol 2022; 13:832928 Hypericum lanceolatum Lam. Medicinal Plant: Potential Toxicity and Therapeutic Effects Based on a Zebrafish Model.
Zachary J Brandt et al. Invest Ophthalmol Vis Sci 2021; 62 (15):13 Ablation of mpeg+ Macrophages Exacerbates mfrp-Related Hyperopia.
Application : IHC-P
Reactivity : Zebrafish
Schmitner N et al. Cells 2021; 10 (11) Differential Responses of Neural Retina Progenitor Populations to Chronic Hyperglycemia.
Application : IHC-Wm
Reactivity : Zebrafish
Vanhunsel S et al. NPJ Aging Mech Dis 2021; 7 (1):22 The killifish visual system as an in vivo model to study brain aging and rejuvenation.
Application : IHC-Fr
Van Houcke J et al. Aging Cell 2021; 20 (9):e13464 Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish Nothobranchius furzeri.
Application : IHC-Fr
Beckers A et al. Neuroscience 2021; 470:52-69 Injury-induced Autophagy Delays Axonal Regeneration after Optic Nerve Damage in Adult Zebrafish.
Application : IHC
Reactivity : Zebrafish
Houcke J.V. et al. bioRxiv 2021; (Epub) Aging impairs the essential contributions of non-glial progenitors to neurorepair in the dorsal telencephalon of the Killifish N. furzeri.
Application : IHC-Fr
Reactivity : Zebrafish
Ping Song et al. Front Cell Dev Biol 2020; 8:578528 Cone Photoreceptor Degeneration and Neuroinflammation in the Zebrafish Bardet-Biedl Syndrome 2 (bbs2) Mutant Does Not Lead to Retinal Regeneration.
Application : IHC-Fr
Reactivity : Zebrafish
Martins RR et al. Front Cell Dev Biol 2019; 7:12 Resident Immunity in Tissue Repair and Maintenance: The Zebrafish Model Coming of Age.
Lahne M et al. Exp Eye Res 2018; 178:148-159 Photo-regulation of rod precursor cell proliferation.
Reactivity : Zebrafish
Lopez-Munoz A et al. Sci Rep 2018; 8 (1):12802 An Adult Zebrafish Model Reveals that Mucormycosis Induces Apoptosis of Infected Macrophages.
Reactivity : Zebrafish
Yang Q et al. Sci Rep 2017; 7 (1):10315 Activation of liver stromal cells is associated with male-biased liver tumor initiation in xmrk and Myc transgenic zebrafish.
Reactivity : Zebrafish
Han P et al. Cell Res 2014; 24 (9):1091-107 Hydrogen peroxide primes heart regeneration with a derepression mechanism.
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169