Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Lin28A antibody [GT717] (GTX628138) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

LIN28 antibody detects LIN28 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min.
Green: LIN28 protein stained by LIN28 antibody (GTX628138) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 ug of whole cell lysate)
A: NT2D1
12% SDS PAGE
GTX628138 diluted at 1:1000

Lin28A antibody detects Lin28A protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Lin28A antibody (GTX628138) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Non-transfected HeLa lysates
B: LIN28 transfected HeLa lysates
12% SDS PAGE
GTX628138 diluted at 1:1000

Lin28A antibody [GT717] detects LIN28 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
12 % SDS-PAGE
Lin28A antibody [GT717] (GTX628138) dilution: 1:1000

Lin28A antibody [GT717] detects Lin28A protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [GT717] dilution: 1:50