Colo320 cell line stained with a cell-line specific M13 phage antibody (filled profile), compared to an irrelevant phage antibody (open profile). Cells were incubated overnight with 1012 phage particles, then detected with 10ug/ml anti-M13 antibody followed by rabbit anti-mouse IgG:FITC

Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.