Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MAN2B2 antibody [C1C3] (GTX120906) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: IMR32
7.5% SDS PAGE
GTX120906 diluted at 1:1000