Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody [GT378] (GTX634471) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

MAP2 antibody [GT378] detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
MAP2 stained by MAP2 antibody [GT378] (GTX634471) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MAP2 antibody [GT378] detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dendrite marker MAP2 stained by MAP2 antibody [GT378] (GTX634471) diluted at 1:1000.
Red: Axon marker Tau, stained by Tau antibody (GTX130462) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).