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MAP2 antibody

Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in Western Blot (WB). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

Cat. No. GTX133109

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Fish
Package
100 μl ($399),
25 μl ($169)
See all MAP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

199 kDa. ( Note )

Positive Control

mouse brain

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse MAP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886830

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule-associated protein 2 , G1-397-34 , MAP-2 , Mtap-2 , Mtap2 , repro4

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite

Database

Research Area

DATA IMAGES

Anti-MAP2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133109

GTX133109 IHC-Fr Image

MAP2 antibody detects MAP2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: MAP2 protein stained by MAP2 antibody (GTX133109) diluted at 1:250.
Red: Tau, stained by Tau antibody (GTX49353) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109

GTX133109 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
MAP2 antibody (GTX133109) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133109

GTX133109 ICC/IF Image

MAP2 antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis .
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Grenn: MAP2 protein stained by MAP2 antibody (GTX133109) diluted at 1:500.
Red (Top right): Dendrites, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Red (Bottom right): Axons, stained by Tau antibody (GTX49353 ) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
MAP2 antibody (GTX133109) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in Western Blot (WB). GTX133109

GTX133109 WB Image

Mouse tissue extract (10 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with MAP2 antibody (GTX133109) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-MAP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX133109

GTX133109 IHC-P Image

MAP2 antibody detects MAP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
MAP2 stained by MAP2 antibody (GTX133109) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

E Partiot et al. EMBO Mol Med 2024; Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studies
Roggeri A et al. Cells 2023; 12 (19) Presynaptic Release-Regulating Sphingosine 1-Phosphate 1/3 Receptors in Cortical Glutamatergic Terminals: Adaptations in EAE Mice and Impact of Therapeutic FTY720.
H Kwon et al. Exp Mol Med 2023; Lactobacillus-derived extracellular vesicles counteract Aβ42-induced abnormal transcriptional changes through the upregulation of MeCP2 and Sirt1 and improve Aβ pathology in Tg-APP/PS1 mice
Jin S et al. Nat Commun 2023; 14 (1) : 5150 Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation.
Hu X et al. Front Cell Neurosci 2022; 16 : 956079 Microglial activation in the motor cortex mediated NLRP3-related neuroinflammation and neuronal damage following spinal cord injury.
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REVIEW

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Application Reference
E Partiot et al. EMBO Mol Med 2024; Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studies
Roggeri A et al. Cells 2023; 12 (19) Presynaptic Release-Regulating Sphingosine 1-Phosphate 1/3 Receptors in Cortical Glutamatergic Terminals: Adaptations in EAE Mice and Impact of Therapeutic FTY720.
H Kwon et al. Exp Mol Med 2023; Lactobacillus-derived extracellular vesicles counteract Aβ42-induced abnormal transcriptional changes through the upregulation of MeCP2 and Sirt1 and improve Aβ pathology in Tg-APP/PS1 mice
Jin S et al. Nat Commun 2023; 14 (1):5150 Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation.
Hu X et al. Front Cell Neurosci 2022; 16:956079 Microglial activation in the motor cortex mediated NLRP3-related neuroinflammation and neuronal damage following spinal cord injury.
Wu SH et al. Biomedicines 2021; 9 (11) Comparison of the Confluence-Initiated Neurogenic Differentiation Tendency of Adipose-Derived and Bone Marrow-Derived Mesenchymal Stem Cells.
Application : ICC/IF
Reactivity : Human
Wu SH et al. Front Cell Dev Biol 2021; 9:658099 Adipose-Derived Mesenchymal Stem Cells From a Hypoxic Culture Improve Neuronal Differentiation and Nerve Repair.
Application : ICC/IF
Reactivity : Human
Wang JP et al. J Tissue Eng Regen Med 2020; 14 (12):1804-1814 Mesenchymal stem cells from a hypoxic culture improve nerve regeneration.
Application : ICC/IF
Reactivity : Human
Wang L et al. J Cereb Blood Flow Metab 2020;:271678X20952264 Remote ischemic conditioning enhances oxygen supply to ischemic brain tissue in a mouse model of stroke: Role of elevated 2,3-biphosphoglycerate in erythrocytes.
Reactivity : Mouse
Ruiz-Palacios M et al. Fish Shellfish Immunol 2020; 106:161-166 Establishment of a brain cell line (SaB-1) from gilthead seabream and its application to fish virology.
Application : ICC/IF
Reactivity : Fish
Zhang J et al. Front Cell Neurosci 2020; 14:195 Mouse Astrocytes Promote Microglial Ramification by Releasing TGF-β and Forming Glial Fibers.
Ruiz-Palacios M et al. Sci Total Environ 2020; 708:134821 Establishment of a brain cell line (FuB-1) from mummichog (Fundulus heteroclitus) and its application to fish virology, immunity and nanoplastics toxicology.
Application : ICC/IF
Nan H et al. J Hum Genet 2019; 64 (11):1055-1065 UBAP1 mutations cause juvenile-onset hereditary spastic paraplegias (SPG80) and impair UBAP1 targeting to endosomes.
Application : ICC/IF
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169